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A novel antimicrobial peptide A2M3 derived from human alpha-2-macroglobulin inhibitsStaphylococcus aureus
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Xuenan FAN1, Rong LIN1, 2, 3, *, Ritian JIN1, 2, 3, Duo LIANG1, 3, Xujian QIU1, 3, Shen YANG1, 2, 3, *
Acta Microbiologica Sinica | 2024, 64(3) : 938 - 952
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Acta Microbiologica Sinica | 2024, 64(3): 938-952
• Research Articles •
A novel antimicrobial peptide A2M3 derived from human alpha-2-macroglobulin inhibitsStaphylococcus aureus
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Xuenan FAN1, Rong LIN1, 2, 3, *, Ritian JIN1, 2, 3, Duo LIANG1, 3, Xujian QIU1, 3, Shen YANG1, 2, 3, *
Affiliations
  • 1 College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, Fujian, China
  • 2 Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Jimei University, Xiamen 361021, Fujian, China
  • 3 Collaborative Innovation Center for Key Technologies of Deep Processing of Marine Food, Dalian Polytechnic University, Dalian 116034, Liaoning, China
Published: 2024-03-04 doi: 10.13343/j.cnki.wsxb.20230637
Outline
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[Objective] To study the inhibitory effect and mechanism of an antimicrobial peptide (A2M3) derived from alpha-2-macroglobulin identified in the human nasal cavity againstStaphylococcus aureus. [Methods] The mass spectrometry results of the human nasal liquid were analyzed, on the basis of which bioinformatic tools were used for the screening of potential antimicrobial peptides. The minimum inhibitory concentration (MIC) and time-kill curve of A2M3 againstS.aureus were determined by the microdilution method and plate colony counting method. Then, transmission electron microscopy, PI uptake assay, flow cytometry, and determination of nucleic acid protein leakage were employed to study the effects of A2M3 on the membrane integrity and permeability ofS.aureus. Finally, the effect of A2M3 on the genomic DNA ofS.aureus was investigated by the gel retardation assay and fluorescence spectroscopy. [Results] A2M3 showed an MIC of 125.0 µg/mL againstS.aureus and killed the bacteria completely within 3 h. A2M3 increased the cell membrane permeability to penetrate intoS.aureus cells, leading to leakage of nucleic acids and proteins as well as insertion into DNA base pairs to interfere with the gene function, resulting in the death of the cells. [Conclusion] The inhibitory mechanism of A2M3 againstS.aureus involves multiple targets. The antimicrobial peptide alters the permeability of the bacterial cell membrane and affects the gene function, thus exerting the inhibitory activity. The findings reveal that antimicrobial peptides could be screened and isolated from human body fluids for potential application.

nasal cavity  /  alpha-2-macroglobulin  /  Staphylococcus aureus  /  antimicrobial peptide  /  antibacterial mechanism
Xuenan FAN, Rong LIN, Ritian JIN, Duo LIANG, Xujian QIU, Shen YANG. A novel antimicrobial peptide A2M3 derived from human alpha-2-macroglobulin inhibitsStaphylococcus aureus[J]. Acta Microbiologica Sinica, 2024 , 64 (3) : 938 -952 . DOI: 10.13343/j.cnki.wsxb.20230637
Funding
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  • Fujian Provincial Department of Science and Technology(2023N5008)
  • Xiamen Science and Technology Subsidy Project(2023CXY0305)
Appendix
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Year 2024 volume 64 Issue 3
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144
62
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Article Info
doi: 10.13343/j.cnki.wsxb.20230637
  • Receive Date：2023-10-17
  • Online Date：2026-03-19
  • Published：2024-03-04
Article Data
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History
  • Received：2023-10-17
  • Accepted：2023-12-20
Funding
Fujian Provincial Department of Science and Technology(2023N5008)
Xiamen Science and Technology Subsidy Project(2023CXY0305)
Affiliations
    1 College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, Fujian, China
    2 Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Jimei University, Xiamen 361021, Fujian, China
    3 Collaborative Innovation Center for Key Technologies of Deep Processing of Marine Food, Dalian Polytechnic University, Dalian 116034, Liaoning, China

Corresponding:

*LIN Rong, E-mail:;
YANG Shen, E-mail:
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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