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[Objective] To isolate the pathogenic bacteria ofDrosophilasuzukii and study the mechanism of bacterial pathogenicity towards the host. [Methods] The pathogenic bacteria were isolated by the plate streaking method and identified based on the 16S rRNA gene sequences. The acidified ethanol method was employed to measure the ability of the isolate to cause browning. The pathogenicity of the isolate was examined by oral infection. Dihydroethidium (DHE) was used to determine the level of reactive oxygen species (ROS), and brilliant blue staining was used to examine the intestinal permeability. [Results] A strain was isolated from the brown culture medium ofD.suzukii and identified asGluconobacter oxydans. The strain reduced the survival rate ofD.suzukii to 49.41% on day 8 (P < 0.001). Furthermore, it weakened the tolerance ofD.suzukii to desiccation and starvation, reducing the survival rate to 58% and 50.9% at the time points of 24 h and 32 h, respectively (P < 0.001). Moreover, the flies treated withG.oxydans displayed impaired integrity of the intestine and had higher level of ROS in the guts than the control group (P < 0.001).G.oxydans robustly reduced the medium pH (pH 2.0), which compromised the survival rate ofD.suzukii and the growth ofLactobacillus plantarum, a probiotic ofD.suzukii. [Conclusion] G.oxydans was a potent pathogen capable of reducing the survival rate ofD.suzukii by lowering the medium pH and inhibiting the growth of probiotics, demonstrating the potential of serving the biocontrol ofD.suzukii.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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