PDF
Full
Outline
[Objective] To investigate the effects and mechanism ofLactobacillus plantarum postbiotics at different doses on amelioratingSalmonellaenterica Typhimurium (ST) infection in mice. [Methods] Sixty 5-week C57BL/6 mice were randomized into five groups: Control, ST, CFS-L+ST, CFS-M+ST, and CFS-H+ST.Lactobacillus plantarum postbiotics (cell-free supernatant, CFS) was administrated at low (L), medium (M), and high (H) doses (50, 100, and 200 μL, respectively) for 21 days. On day 22, mice were orally challenged with ST at 3×108 CFU, and the samples were collected three days later. [Results] Compared with the control group, CFS-L+ST and CFS-M+ST groups showed no significant changes in body weight gain, while the CFS-H+ST group showed a significant decrease (P<0.05). The CFS-M+ST and CFS-H+ST groups alleviated ST-induced body weight loss (P<0.05). CFS pretreatment reduced ST-induced bacterial translocation in the colon, liver, spleen, and brain (P<0.05) and alleviated the pathological damages in the colon and spleen. ST reduced the levels of acetic acid and butyric acid in the cecum, which, however, increased in the CFS-M+ST group (P>0.05). Compared with the ST group, CFS-M+ST alleviated the inflammatory response by lowering the levels of pro-inflammatory cytokines including interleukin-1beta (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) (P<0.05) and elevating the levels of anti-inflammatory cytokines including interleukin-4 (IL-4) and interleukin-10 (IL-10) (P<0.05). Moreover, CFS-M+ST suppressed ST-induced inflammation by modulating the nucleotide-binding oligomerization domain-like receptor (NLR) family pyrin domain-containing protein 3 (NLRP3) inflammasome, as indicated by the down-regulated mRNA levels ofNLRP3, apoptosis-associated speck-like protein (ASC), cysteine-dependent aspartate-specific protease 1 (caspase-1), and gasdermin D (GSDMD) (P<0.05). Furthermore, CFS inhibited NLRP3 inflammasome by blocking the upstream key nuclear factor kappa beta (NF-κB) pathway, as indicated by the down-regulated expression levels of myeloid differentiation factor 88 (MyD88), tumor necrosis factor receptor-associated factor 6 (TRAF6), transforming growth factor beta-activated kinase 1 (TAK1), and NF-κB (P<0.05). [Conclusion] L.plantarum postbiotics CFS alleviated theS.enterica Typhimurium infection and inflammatory responses in mice by inhibiting the NF-κB-mediated NLRP3 inflammasome, and the pretreatment with medium-dose CFS showed the best effects.
PDF
112
50
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
