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Functions of the aconitase family inFusarium proliferatum
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Yizhou GAO, Siming HE, Yitong WANG, Haibo LI, Yuqing WANG, Zhihong WU*
Acta Microbiologica Sinica | 2024, 64(4) : 1306 - 1321
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Acta Microbiologica Sinica | 2024, 64(4): 1306-1321
• Research Articles •
Functions of the aconitase family inFusarium proliferatum
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Yizhou GAO, Siming HE, Yitong WANG, Haibo LI, Yuqing WANG, Zhihong WU*
Affiliations
  • School of Biological and Chemical Engineering, Zhejiang University of Science & Technology, Hangzhou 310023, Zhejiang, China
Published: 2024-04-04 doi: 10.13343/j.cnki.wsxb.20230729
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[Objective] Fusarium proliferatum is one of the major pathogens causing root rot of alfalfa. This study aims to investigate the function of the aconitase family inF.proliferatum and give insights into the molecular mechanisms underlying the physiological metabolism of this pathogen. [Methods] We employed the hmmsearch tool to identify the proteins containing the aconitase domain inF.proliferatum and then carried out the phylogenetic analysis. real-time PCR and SWISS-MODEL were employed to analyze the expression profiles ofFpACO genes and the protein structures, respectively. The homologous recombination method was used to construct theFpACO-deleted mutants ofF.proliferatum. Furthermore, we explored the growth, sporulation, spore morphology, stress responses, and pathogenicity of ΔFpACO3, ΔFpACO4-1, and ΔFpACO4-2 and measured the mitochondrial metabolism indicators of the mutants. [Results] FpACO4-1 and FpACO4-2 were involved in sporulation and spore morphogenesis. FpACO3, FpACO4-1, and FpACO4-2 were responsible for regulating the sensitivity ofF.proliferatum to cell wall stress and metal ion stress. Moreover, FpACO3, FpACO4-1, and FpACO4-2 affected the mitochondrial metabolism indicators, including the total aconitase activity, the ATP level, the hydrogen peroxide level, and the expression of key genes in the tricarboxylic acid cycle. [Conclusion] The aconitase family members are involved in the regulation of the processes such as sporulation, spore morphogenesis, response to cell wall stress and metal ion stress, and mitochondrial metabolism inF.proliferatum.

Fusarium proliferatum  /  root rot of alfalfa  /  aconitase  /  mitochondrial metabolism
Yizhou GAO, Siming HE, Yitong WANG, Haibo LI, Yuqing WANG, Zhihong WU. Functions of the aconitase family inFusarium proliferatum[J]. Acta Microbiologica Sinica, 2024 , 64 (4) : 1306 -1321 . DOI: 10.13343/j.cnki.wsxb.20230729
Funding
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  • Scientific Research Foundation of Zhejiang University of Science & Technology(F701103M09)
  • General Scientific Research Project from the Education Department of Zhejiang Province(Y202351867)
Appendix
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Year 2024 volume 64 Issue 4
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Article Info
doi: 10.13343/j.cnki.wsxb.20230729
  • Receive Date：2023-11-29
  • Online Date：2026-03-19
  • Published：2024-04-04
Article Data
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History
  • Received：2023-11-29
  • Accepted：2024-01-25
Funding
Scientific Research Foundation of Zhejiang University of Science & Technology(F701103M09)
General Scientific Research Project from the Education Department of Zhejiang Province(Y202351867)
Affiliations
    School of Biological and Chemical Engineering, Zhejiang University of Science & Technology, Hangzhou 310023, Zhejiang, China

Corresponding:

*WU Zhihong, E-mail:
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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