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Application of high-throughput technology in the screening of terpene synthases
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Bei LI1, 2, Zixin DENG1, Min XU1, *, Anwei HOU1, 3, *
Acta Microbiologica Sinica | 2024, 64(2) : 408 - 431
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Acta Microbiologica Sinica | 2024, 64(2): 408-431
• Reviews •
Application of high-throughput technology in the screening of terpene synthases
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Bei LI1, 2, Zixin DENG1, Min XU1, *, Anwei HOU1, 3, *
Affiliations
  • 1 Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China
  • 2 Haihe Laboratory of Synthetic Biology, Tianjin 300308, China
  • 3 Institute of Microbiology, Jiangxi Academy of Sciences, Nanchang 330096, Jiangxi, China
Published: 2024-02-04 doi: 10.13343/j.cnki.wsxb.20230518
Outline
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Terpenoids, a group of natural products possessing diverse chemical structures and biological activities, have been widely used in the production of food additives, medicines, and cosmetics. Terpenoids are mainly produced by plants. With the rapid development of synthetic biology, heterologous production of terpenoids by engineered microbial strains is more economical and environmentally friendly compared with the conventional methods of extraction from plants and chemical synthesis. The catalytic activities of terpene synthases and the structural specificity of their products are pivotal for the heterologous biosynthesis of terpenoids. Directed evolution and rational design can be adopted to optimize the catalytic properties and product profile of target terpene synthases. However, this requires a feasible high-throughput method to screen mutant libraries. In recent years, various high-throughput screening methods have been developed to boost the sensitivity and efficiency in screening terpene synthases. We review recently established high-throughput screening methods for terpene synthases and briefly outline the principles and advantages and disadvantages of each method. Eventually, we envision the future directions of the application of high-throughput screening in the engineering of terpene synthases.

high-throughput screening  /  terpene synthase  /  directed evolution  /  terpenoid
Bei LI, Zixin DENG, Min XU, Anwei HOU. Application of high-throughput technology in the screening of terpene synthases[J]. Acta Microbiologica Sinica, 2024 , 64 (2) : 408 -431 . DOI: 10.13343/j.cnki.wsxb.20230518
Funding
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  • National Natural Science Foundation of China(22107038)
  • Major Project and Innovation Fund of the Haihe Laboratory of Synthetic Biology(22HHSWSS00001)
  • Major Project and Innovation Fund of the Haihe Laboratory of Synthetic Biology(22HHSWSS00007)
  • Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project(TSBICIP-CXRC-065)
  • Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project(TSBICIP-CXRC-076)
  • Project of Jiangxi Academy of Sciences(2018-XTPH1-02)
  • Project of Jiangxi Academy of Sciences(2018-YYB-02)
Appendix
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Year 2024 volume 64 Issue 2
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160
67
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Article Info
doi: 10.13343/j.cnki.wsxb.20230518
  • Receive Date：2023-08-09
  • Online Date：2026-03-18
  • Published：2024-02-04
Article Data
Affiliations
History
  • Received：2023-08-09
  • Accepted：2023-11-02
Funding
National Natural Science Foundation of China(22107038)
Major Project and Innovation Fund of the Haihe Laboratory of Synthetic Biology(22HHSWSS00001)
Major Project and Innovation Fund of the Haihe Laboratory of Synthetic Biology(22HHSWSS00007)
Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project(TSBICIP-CXRC-065)
Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project(TSBICIP-CXRC-076)
Project of Jiangxi Academy of Sciences(2018-XTPH1-02)
Project of Jiangxi Academy of Sciences(2018-YYB-02)
Affiliations
    1 Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China
    2 Haihe Laboratory of Synthetic Biology, Tianjin 300308, China
    3 Institute of Microbiology, Jiangxi Academy of Sciences, Nanchang 330096, Jiangxi, China

Corresponding:

*E-mail: HOU Anwei,;
E-mail: XU Min,
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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