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Functions of genes encoding phosphomevalonate kinase and mevalonate diphosphate decarboxylase inCordyceps militaris
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Huanhuan YAN1, 2, Yitong SHANG1, 2, Lihong WANG1, 2, Xueqin TIAN1, 2, Lihua YAO1, Bin ZENG2, 3, *, Zhihong HU1, 2, *
Acta Microbiologica Sinica | 2024, 64(2) : 461 - 472
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Acta Microbiologica Sinica | 2024, 64(2): 461-472
• Research Articles •
Functions of genes encoding phosphomevalonate kinase and mevalonate diphosphate decarboxylase inCordyceps militaris
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Huanhuan YAN1, 2, Yitong SHANG1, 2, Lihong WANG1, 2, Xueqin TIAN1, 2, Lihua YAO1, Bin ZENG2, 3, *, Zhihong HU1, 2, *
Affiliations
  • 1 College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang 330013, Jiangxi, China
  • 2 Jiangxi Key Laboratory of Bioprocess Engineering, Jiangxi Science and Technology Normal University, Nanchang 330013, Jiangxi, China
  • 3 College of Pharmacy, Shenzhen Technology University, Shenzhen 518118, Guangdong, China
Published: 2024-02-04 doi: 10.13343/j.cnki.wsxb.20230345
Outline
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[Objective] To determine the functions of two key enzymes of the mevalonate pathway, phosphomevalonate kinase (CmErg8) and mevalonate diphosphate decarboxylase (CmErg19), inCordyceps militaris and their effects on the content of ergosterol and cordycepin.[Methods] Bioinformatics analysis was conducted to identifyCmErg8 andCmErg19 inC.militaris, and yeast complementation was employed to determine whether their functions were conserved. Furthermore, we employedAgrobacterium tumefaciens-mediated transformation to overexpressCmErg8 andCmErg19 in the auxotrophic mutantCmΔpyrG ofC.militaris, so as to observe the effects ofCmErg8 andCmErg19 on the content of ergosterol and cordycepin.[Results] CmErg8 andCmErg19 could not complement the temperature sensitivity of theerg8 anderg19 mutants of yeast. The strains overexpressingCmErg8 andCmErg19 showed increased content of ergosterol and cordycepin. Particularly, the cordycepin content increased by about 5 times in the strain overexpressingCmErg19 compared with that in the control.[Conclusion] This study revealed the functions ofCmErg8 andCmErg19 inC.militaris and reported that the genes in the ergosterol synthesis pathway ofC.militaris affected the content of cordycepin for the first time.

Cordyceps militaris  /  phosphomevalonate kinase  /  mevalonate diphosphate decarboxylase  /  ergosterol  /  cordycepin
Huanhuan YAN, Yitong SHANG, Lihong WANG, Xueqin TIAN, Lihua YAO, Bin ZENG, Zhihong HU. Functions of genes encoding phosphomevalonate kinase and mevalonate diphosphate decarboxylase inCordyceps militaris[J]. Acta Microbiologica Sinica, 2024 , 64 (2) : 461 -472 . DOI: 10.13343/j.cnki.wsxb.20230345
Funding
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  • Natural Science Foundation of Jiangxi Provincial Department of Science and Technology(20212BAB205001)
  • National Natural Science Fundation of China(32260009)
  • National Natural Science Fundation of China(31960193)
  • "Double Thousand Plan" in Jiangxi Province(jxsq2019201011)
Appendix
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Year 2024 volume 64 Issue 2
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127
52
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Article Info
doi: 10.13343/j.cnki.wsxb.20230345
  • Receive Date：2023-05-15
  • Online Date：2026-03-18
  • Published：2024-02-04
Article Data
Affiliations
History
  • Received：2023-05-15
  • Accepted：2023-11-17
Funding
Natural Science Foundation of Jiangxi Provincial Department of Science and Technology(20212BAB205001)
National Natural Science Fundation of China(32260009)
National Natural Science Fundation of China(31960193)
"Double Thousand Plan" in Jiangxi Province(jxsq2019201011)
Affiliations
    1 College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang 330013, Jiangxi, China
    2 Jiangxi Key Laboratory of Bioprocess Engineering, Jiangxi Science and Technology Normal University, Nanchang 330013, Jiangxi, China
    3 College of Pharmacy, Shenzhen Technology University, Shenzhen 518118, Guangdong, China

Corresponding:

*E-mail: ZENG Bin,;
E-mail: HU Zhihong,
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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