Home Journals Articles Updates About
CN
image
收藏切换
Identification and functional characterization of phytochrome genes in the filamentous fungusPodospora anserina
收藏切换
PDF
Siyu CHEN1, Xiao LI2, Wenzhen DU1, Yuehua GENG3, Gang LIU1, Ning XIE1, *
Acta Microbiologica Sinica | 2024, 64(2) : 443 - 460
Less
收藏切换
Acta Microbiologica Sinica | 2024, 64(2): 443-460
• Research Articles •
Identification and functional characterization of phytochrome genes in the filamentous fungusPodospora anserina
Full
Siyu CHEN1, Xiao LI2, Wenzhen DU1, Yuehua GENG3, Gang LIU1, Ning XIE1, *
Affiliations
  • 1 Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518071, Guangdong, China
  • 2 Editorial Office of Journal of Shenzhen University (Science & Engineering), Shenzhen University, Shenzhen 518060, Guangdong, China
  • 3 College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, Henan, China
Published: 2024-02-04 doi: 10.13343/j.cnki.wsxb.20230138
Outline
收藏切换

Phytochromes play a key role in bacterial and plant development, while their roles in fungi are not fully understood.

[Objective] To explore the roles of phytochrome genesPaPhy1 andPaPhy2 in the sexual reproduction and asexual development ofPodospora anserina and decipher the regulatory mechanisms.[Methods] The homologous recombination method was employed to knock out the two phytochrome genes, and the resulting mutants, ΔPaPhy1 and ΔPaPhy2, were obtained. A double mutant, ΔPaPhy1ΔPaPhy2, was constructedvia genetic cross. We compared the sexual production, asexual development, growth rate, and reactive oxygen metabolism between the mutants and wild-type strains under different light conditions to reveal the main roles of the phytochrome genes inP.anserina.[Results] Blue light and white light induced the formation ofP.anserina ascospore shells. ΔPaPhy produced fewer ascospores in the light and showed prolonged life cycle.[Conclusion] Phytochrome genes are associated with the sexual reproduction ofP.anserina. The aging delay of ΔPaPhy is related to reactive oxygen metabolism. The results of this study provide new ideas for further exploring the mechanism of light in regulating the reproduction of filamentous fungi as well as anti-aging studies.

filamentous fungus  /  Podospora anserina  /  phytochrome  /  gene knockout  /  sexual reproduction  /  aging  /  reactive oxygen metabolism
Siyu CHEN, Xiao LI, Wenzhen DU, Yuehua GENG, Gang LIU, Ning XIE. Identification and functional characterization of phytochrome genes in the filamentous fungusPodospora anserina[J]. Acta Microbiologica Sinica, 2024 , 64 (2) : 443 -460 . DOI: 10.13343/j.cnki.wsxb.20230138
Funding
Less
  • National Key Research and Development Program of China(2021YFA0910800)
  • Natural Science Foundation of Guangdong Province(2021A1515012166)
  • Stability Support Project for Universities in Shenzhen(20200812173625001)
Appendix
Less
Year 2024 volume 64 Issue 2
PDF
143
63
Cite this Article
BibTeX
Article Info
doi: 10.13343/j.cnki.wsxb.20230138
  • Receive Date：2023-03-05
  • Online Date：2026-03-18
  • Published：2024-02-04
Article Data
Affiliations
History
  • Received：2023-03-05
  • Accepted：2023-10-31
Funding
National Key Research and Development Program of China(2021YFA0910800)
Natural Science Foundation of Guangdong Province(2021A1515012166)
Stability Support Project for Universities in Shenzhen(20200812173625001)
Affiliations
    1 Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518071, Guangdong, China
    2 Editorial Office of Journal of Shenzhen University (Science & Engineering), Shenzhen University, Shenzhen 518060, Guangdong, China
    3 College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, Henan, China

Corresponding:

*XIE Ning, Tel/Fax: +86-755-86955803, E-mail:
References
Share
https://castjournals.cast.org.cn/joweb/wswxb/EN/10.13343/j.cnki.wsxb.20230138
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House