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Immobilization of mixed white rot fungi and it application in the treatment of lead-contaminated wastewater
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Xuanwen MAO, Yiwen LI, Xiaoyu JIANG, Yanhe WANG, Nan WU, Yunjie JIANG, Yue LIU, Wei YOU, Tingshu WANG, Yincui XIAO, Fang FANG, Peng LIU*
Acta Microbiologica Sinica | 2024, 64(1) : 283 - 302
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Acta Microbiologica Sinica | 2024, 64(1): 283-302
Research Articles
Immobilization of mixed white rot fungi and it application in the treatment of lead-contaminated wastewater
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Xuanwen MAO, Yiwen LI, Xiaoyu JIANG, Yanhe WANG, Nan WU, Yunjie JIANG, Yue LIU, Wei YOU, Tingshu WANG, Yincui XIAO, Fang FANG, Peng LIU*
Affiliations
  • Botany Laboratory, College of Life Sciences, Zhejiang Normal University, Jinhua 321004, Zhejiang, China
Published: 2024-01-04 doi: 10.13343/j.cnki.wsxb.20230427
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[Objective] To mitigate the threat of heavy metal pollution in wastewater to global food safety and human health, reduce the accumulation of lead (Pb) in soil, plants, and animals, and improve the removal rate of heavy metals by immobilizing microbial strains.[Methods] We carried out mixed strain test to select the white rot fungal strains with strong Pb2+ removal effects and excellent compatibility and explored the optimal strains and ratio for combination. Furthermore, we optimized the formula of the fungal strain composite and explored the optimal adsorption conditions of the composite in application.[Results] Phanerochaete chrysosporium,Coriolus versicolor,Lentinus sajor-caju, andPleurotus ostreatus with good compatibility were selected for subsequent experiments.C.versicolor mixed withL.sajor-caju at a volume ratio of 1:1 outperformed the single strains in removing Pb2+. The fungal strain composite composed of 20.0 g/L sodium alginate, 15.0 g/L biochar, 2.0×106 CFU/mL white rot fungi, silica, and zeolite showed the Pb2+ removal rate of 90.63% within 96 h. Moreover, this composite had higher mechanical strength and strong resistance to mechanical shear. At the addition amount of 8.35 g/L and pH 5.64, the composite demonstrated the Pb2+ removal rate of 97.45% within 96 h. Moreover, this composite can be reused 7 times after adsorption-desorption-readsorption and maintained high Pb2+ removal capacity.[Conclusion] The immobilized white rot fungal strain composite can significantly improve the microbial utilization rate and wastewater treatment efficiency compared with single strains. It can greatly adsorb Pb2+ in wastewater under appropriate conditions within a short time and reduce the environmental threat caused by heavy metal pollutants. Therefore, the promotion of environmental protection greatly benefits from the use of immobilized mixed white rot fungal strains in the treatment of heavy metal-contaminated wastewater.

lead pollution  /  white rot fungi  /  immobilization of mixed fungi  /  component variations of extracellular polymeric substances
Xuanwen MAO, Yiwen LI, Xiaoyu JIANG, Yanhe WANG, Nan WU, Yunjie JIANG, Yue LIU, Wei YOU, Tingshu WANG, Yincui XIAO, Fang FANG, Peng LIU. Immobilization of mixed white rot fungi and it application in the treatment of lead-contaminated wastewater[J]. Acta Microbiologica Sinica, 2024 , 64 (1) : 283 -302 . DOI: 10.13343/j.cnki.wsxb.20230427
  • National Natural Science Foundation of China(32001224)
  • National Natural Science Foundation of China(41702181)
  • Jinhua City Science and Technology Plan Public Welfare Project(2022-4-049)
Year 2024 volume 64 Issue 1
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Article Info
doi: 10.13343/j.cnki.wsxb.20230427
  • Receive Date:2023-06-20
  • Online Date:2026-03-18
  • Published:2024-01-04
Article Data
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History
  • Received:2023-06-20
  • Accepted:2023-08-29
Funding
National Natural Science Foundation of China(32001224)
National Natural Science Foundation of China(41702181)
Jinhua City Science and Technology Plan Public Welfare Project(2022-4-049)
Affiliations
    Botany Laboratory, College of Life Sciences, Zhejiang Normal University, Jinhua 321004, Zhejiang, China

Corresponding:

*LIU Peng, E-mail:
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表12种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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