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BldM regulates morphological development and antibiotic synthesis inStreptomyces pactum Act12
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Yuan ZHANG1, Hanqi ZHOU1, Kangkang ZHAO1, Quanhong XUE2, Lianghui JIA1, Xia YAN1, *, Hua YAN1, *
Acta Microbiologica Sinica | 2024, 64(1) : 130 - 142
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Acta Microbiologica Sinica | 2024, 64(1): 130-142
• Research Articles •
BldM regulates morphological development and antibiotic synthesis inStreptomyces pactum Act12
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Yuan ZHANG1, Hanqi ZHOU1, Kangkang ZHAO1, Quanhong XUE2, Lianghui JIA1, Xia YAN1, *, Hua YAN1, *
Affiliations
  • 1 College of Life Sciences, Northwest A & F University, Yangling 712100, Shaanxi, China
  • 2 College of Natural Resources and Environment, Northwest A & F University, Yangling 712100, Shaanxi, China
Published: 2024-01-04 doi: 10.13343/j.cnki.wsxb.20230312
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[Objective] To investigate the regulatory role of the transcription factor BldM in the morphological development and antibiotic synthesis ofStreptomyces pactum Act12, a biocontrol strain with multiple effects.[Methods] ThebldM-deleted mutant strain ∆bldM and thebldM-overexpressing mutant strain OE-bldM were constructed by genetic engineering. The scanning electron microscopy, antibacterial experiment, high performance liquid chromatography, and real-time quantitative PCR were employed to compare the morphological development, growth rate, oligomycin yield, and resistance to pathogens, respectively, between ∆bldM, OE-bldM, and the wild-type strain Act12.[Results] The sequencing results proved that ∆bldM and OE-bldM were successfully constructed. ∆bldM showed significantly reduced production of oligomycin D and was incapable of forming aerial hyphae. OE-bldM presented dense aerial hyphae and active sporulation. Compared with the wild type, OE-bldM showed an increase of 23% in the yield of oligomycin D and the up-regulation of 2–3 times in the transcriptional levels of the genes encoding oligomycin core synthetase. Moreover, the antimicrobial activity of OE-bldM remarkably enhanced.[Conclusion] The global transcriptional regulator BldM can not only affect the formation of aerial hyphae and sporulation but also participate in the positive regulation of oligomycin synthesis in Act12. The results of this study supplement the knowledge about the regulatory function of BldM and provide a reference for further research on the growth, metabolism, and regulation mechanism ofS.pactum Act12.

Streptomyces pactum Act12  /  oligomycin  /  bldM  /  morphological development
Yuan ZHANG, Hanqi ZHOU, Kangkang ZHAO, Quanhong XUE, Lianghui JIA, Xia YAN, Hua YAN. BldM regulates morphological development and antibiotic synthesis inStreptomyces pactum Act12[J]. Acta Microbiologica Sinica, 2024 , 64 (1) : 130 -142 . DOI: 10.13343/j.cnki.wsxb.20230312
Funding
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  • National Natural Science Foundation of China(31601700)
  • National Natural Science Foundation of China(32072477)
  • Natural Science Basic Research Program of Shaanxi Province(2021JM-105)
Appendix
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Year 2024 volume 64 Issue 1
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152
59
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Article Info
doi: 10.13343/j.cnki.wsxb.20230312
  • Receive Date：2023-05-04
  • Online Date：2026-03-18
  • Published：2024-01-04
Article Data
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History
  • Received：2023-05-04
  • Accepted：2023-09-13
Funding
National Natural Science Foundation of China(31601700)
National Natural Science Foundation of China(32072477)
Natural Science Basic Research Program of Shaanxi Province(2021JM-105)
Affiliations
    1 College of Life Sciences, Northwest A & F University, Yangling 712100, Shaanxi, China
    2 College of Natural Resources and Environment, Northwest A & F University, Yangling 712100, Shaanxi, China

Corresponding:

*YAN Xia, E-mail:;
YAN Hua, E-mail:
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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