PDF
Full
Outline
[Objective] To screen out a fungal strain that can efficiently assimilate ammonia nitrogen, reveal the metabolome differences of the strain in different media and the changes in the amino acid content of the feed fermented with the strain, and clarify the mechanism of its ammonia assimilation.[Methods] Seven strains ofTrichoderma, 7 strains ofAspergillus niger, and 9 strains ofAspergillus oryzae were cultured in the media with (NH4)2SO4 as the only nitrogen source. The strains with high ammonia nitrogen use efficiency and glutamine synthetase (GS) activity were selected for comparison of the metabolic differences in potato dextrose agar (PDA) plates and inorganic nitrogen plates by non-targeted metabonomics. Furthermore, the crude protein and organic nitrogen content in the feed fermented with different strains was determined, and the changes in amino acid content in the fermented feed extract were measured by amino acid-targeted metabolomics.[Results] The utilization rate of ammonia nitrogen and the glutamine synthetase activity ofA. oryzae MQ28 were 54.46% and 0.61 μmol/(h·g), respectively, which were higher than those of other strains (P < 0.05). The comparative metabonomics analysis suggested that MQ28 was associated with the metabolism of multiple amino acids during ammonia assimilation. MQ28 fermentation increased the crude protein and organic nitrogen in the feed by 22.25% and 35.83% (P < 0.05), respectively. Furthermore, MQ28 fermentation increased the total amino acid content in feed extract from 31.86 mmol/100 g to 57.69 mmol/100 g (P < 0.05). Specifically, it increased the content of 14 amino acids such as threonine, lysine, and arginine, glutamic acid (by 3.46 folds), and glutamine (by 99 folds) (P < 0.05).[Conclusion] To sum up,A.oryzae MQ28 has high ammonia nitrogen utilization capacity. It may regulate the ammonia assimilation process through the synthesis of glutamine to regulate amino acid metabolism and can be used as an elite strain for the production of single-cell protein.
PDF
105
44
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
