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Key amino acid sites of the TetR family transcription factor BcPDR1 in Botrytis cinerea
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Dexuan QU1, 2, Xiaoying LIU1, 2, Yadi WEI1, Jinping ZANG1, Hongzhe CAO1, Kang ZHANG1, 2, Jihong XING1, 2, *, Jingao DONG1, 2, *
Acta Microbiologica Sinica | 2026, 66(3) : 1107 - 1118
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Acta Microbiologica Sinica | 2026, 66(3): 1107-1118
• Research Article •
Key amino acid sites of the TetR family transcription factor BcPDR1 in Botrytis cinerea
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Dexuan QU1, 2, Xiaoying LIU1, 2, Yadi WEI1, Jinping ZANG1, Hongzhe CAO1, Kang ZHANG1, 2, Jihong XING1, 2, *, Jingao DONG1, 2, *
Affiliations
  • 1.Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, Baoding, Hebei, China
  • 2.State Key Laboratory of North China Crop Improvement and Regulation, Baoding, Hebei, China
Published: 2026-03-04 doi: 10.13343/j.cnki.wsxb.20250707
Outline
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Objective To identify the key amino acid residues of the TetR family transcription factor BcPDR1 in Botrytis cinerea, thereby laying a foundation for elucidating the mechanism by which BcPDR1 regulates the growth, development, and pathogenicity of this pathogen. Methods The key amino acid sites of BcPDR1 were analyzed by bioinformatics methods, and four conserved regions (32-34 aa, 76-95 aa, 140-150 aa, and 189 aa) were selected for site-directed mutagenesis. On the basis of the knockout mutant ΔBcpdr1, the mutants BcPDR1-M1 (Δ32-34), BcPDR1-M2 (Δ76-95), BcPDR1-M3 (Δ140-150), and BcPDR1-M4 (mutation of Ile to Lys at 189 aa) were constructed. A comparative analysis of the phenotypic characteristics and pathogenicity was conducted on the four aforementioned mutants and the wild-type strain of B. cinerea, ΔBcpdr1, the complemented strain CE. Results The colony morphology, mycelial morphology, and growth rates of BcPDR1-M1, BcPDR1-M2, BcPDR1-M3, and BcPDR1-M4 were similar to those of ΔBcpdr1, but significantly different from those of BC22 and CE. These mutants could form lesions on tomato fruits and tobacco leaves, while their lesion areas were significantly smaller than those of BC22 and CE. Conclusion The regions 32-34, 76-95, 140-150, and the 189th amino acid are the regulatory sites for BcPDR1 to exert its functions.

Botrytis cinerea  /  BcPDR1  /  key amino acid sites  /  growth and development  /  pathogenicity
Dexuan QU, Xiaoying LIU, Yadi WEI, Jinping ZANG, Hongzhe CAO, Kang ZHANG, Jihong XING, Jingao DONG. Key amino acid sites of the TetR family transcription factor BcPDR1 in Botrytis cinerea[J]. Acta Microbiologica Sinica, 2026 , 66 (3) : 1107 -1118 . DOI: 10.13343/j.cnki.wsxb.20250707
Funding
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  • National Natural Science Foundation of China(32072369)
  • Central Guidance for Local Technology Development Funding(246Z6506G)
Appendix
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Year 2026 volume 66 Issue 3
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153
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Article Info
doi: 10.13343/j.cnki.wsxb.20250707
  • Receive Date：2025-09-16
  • Online Date：2026-03-12
  • Published：2026-03-04
Article Data
Affiliations
History
  • Received：2025-09-16
  • Accepted：2025-10-28
Funding
National Natural Science Foundation of China(32072369)
Central Guidance for Local Technology Development Funding(246Z6506G)
Affiliations
    1.Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, Baoding, Hebei, China
    2.State Key Laboratory of North China Crop Improvement and Regulation, Baoding, Hebei, China

Corresponding:

*E-mail: XING Jihong,
DONG Jingao,
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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