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Expression and functional characterization of UrcA-like family membrane protein from Sphingobium xenophagum
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Mingyue WANG, Xiaodan ZHENG, Hui YAO, Meiying XU*, Xingjuan CHEN*
Acta Microbiologica Sinica | 2026, 66(3) : 1192 - 1210
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Acta Microbiologica Sinica | 2026, 66(3): 1192-1210
• Research Article •
Expression and functional characterization of UrcA-like family membrane protein from Sphingobium xenophagum
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Mingyue WANG, Xiaodan ZHENG, Hui YAO, Meiying XU*, Xingjuan CHEN*
Affiliations
  • State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, Guangdong, China
Published: 2026-03-04 doi: 10.13343/j.cnki.wsxb.20250759
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Objective To analyze the expression strategy, DNA-binding characteristics, and the role in heavy metal responses and transcriptional regulation of the UrcA-like membrane protein Chr1_2170 from Sphingobium xenophagum C1. Methods Chr1_2170 was expressed in Escherichia coli BL21(DE3) by codon optimization, dual-signal peptide guidance, and co-expression with homologous molecular chaperones. The interacting genes of Chr1_2170 were screened by constructing a functional promoter library of protein-bound genomic DNA fragments. The heavy metal response characteristics of Chr1_2170 were analyzed via the Chr1_2170-Luc reporter system. Results Chr1_2170 was successfully expressed in E. coli BL21(DE3). Six promoter regions specifically bound by Chr1_2170 were screened out and identified, with the conserved motif of 5′-AATXGCGXGTA-3′. Gene function annotation predicted that Chr1_2170 regulated multiple genes, including those encoding β-N-acetylglucosaminidase, two-component system ATP-binding protein, DNA topoisomerase IV subunit B, and serine hydrolase. Chr1_2170 showed dose-dependent responses to Cu2+ (1-80 μmol/L), Zn2+ (1-80 μmol/L), and Ba2+ (1-150 μmol/L). Conclusion Chr1_2170 functions not only as a heavy metal sensing element but also as a multifunctional transcriptional regulator. It regulates the expression of related genes by recognizing specific DNA sequences, playing a key role in environmental adaptation and stress responses of bacteria.

Sphingobium  /  UrcA family protein  /  membrane protein expression  /  transcriptional regulation  /  heavy metal response
Mingyue WANG, Xiaodan ZHENG, Hui YAO, Meiying XU, Xingjuan CHEN. Expression and functional characterization of UrcA-like family membrane protein from Sphingobium xenophagum[J]. Acta Microbiologica Sinica, 2026 , 66 (3) : 1192 -1210 . DOI: 10.13343/j.cnki.wsxb.20250759
Funding
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  • National Key Research and Development Program of China(2021YFA0910300)
  • National Natural Science Foundation of China(32171409)
  • Key Research and Development Program of Guangdong Province(2023B0202040001)
  • Guangdong Special Support Plan Outstanding Talents Project(2023JC07L096)
  • GDAS Project of Science and Technology Development(2022GDASZH-2022010101)
Appendix
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Year 2026 volume 66 Issue 3
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156
62
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Article Info
doi: 10.13343/j.cnki.wsxb.20250759
  • Receive Date：2025-10-10
  • Online Date：2026-03-12
  • Published：2026-03-04
Article Data
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History
  • Received：2025-10-10
  • Accepted：2025-11-10
Funding
National Key Research and Development Program of China(2021YFA0910300)
National Natural Science Foundation of China(32171409)
Key Research and Development Program of Guangdong Province(2023B0202040001)
Guangdong Special Support Plan Outstanding Talents Project(2023JC07L096)
GDAS Project of Science and Technology Development(2022GDASZH-2022010101)
Affiliations
    State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, Guangdong, China

Corresponding:

*E-mail: CHEN Xingjuan,
XU Meiying,
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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