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Characterization of the first penicillin-resistant Streptococcus orisratti strain carrying both ermB and optrA in China
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Haowen TAI1, Jiaming ZHANG1, Han ZHENG1, Yuqing LIU1, 2, Jingyi ZHANG1, Huiqiang FU1, Yifan ZHANG1, Juan LI1, *
Acta Microbiologica Sinica | 2026, 66(3) : 1311 - 1325
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Acta Microbiologica Sinica | 2026, 66(3): 1311-1325
• Research Article •
Characterization of the first penicillin-resistant Streptococcus orisratti strain carrying both ermB and optrA in China
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Haowen TAI1, Jiaming ZHANG1, Han ZHENG1, Yuqing LIU1, 2, Jingyi ZHANG1, Huiqiang FU1, Yifan ZHANG1, Juan LI1, *
Affiliations
  • 1.National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China
  • 2.National Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China
Published: 2026-03-04 doi: 10.13343/j.cnki.wsxb.20250787
Outline
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Objective To perform biological characterization and genomic analysis for a multidrug-resistant Streptococcus strain isolated from the tonsils of a dead piglet, thus elucidating its phenotypic and genotypic characteristics. Methods The tonsillar tissue homogenate from the dead piglet was inoculated into the Streptococcus enrichment medium for enrichment culture. The enriched culture was then streaked onto Columbia blood agar medium for Streptococcus isolation. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), a biochemical identification system, and 16S rRNA gene sequencing were employed for strain identification. The minimum inhibitory concentrations (MICs) were determined by the broth microdilution method. Whole-genome sequencing was conducted via the Illumina platform. The ResFinder database was used to analyze the resistance genes, and mutations in the genes encoding penicillin-binding proteins (PBPs) were investigated through multiple sequence alignment. Results The phylogenetic analysis based on 16S rRNA gene sequences identified the isolate as Streptococcus orisratti. Antimicrobial susceptibility testing revealed that the strain was resistant to penicillin G, erythromycin, clindamycin, and compound sulfamethoxazole, and it exhibited reduced susceptibility to linezolid. Whole-genome analysis identified the presence of the macrolide and lincoamide resistance gene ermB and the oxazolidinone resistance gene optrA. Furthermore, multiple mutations were detected in the genes encoding PBPs. Conclusion This study reports the first isolate of S. orisratti harboring both ermB and optrA and exhibiting resistance to penicillin. It highlights the capacity of porcine-derived streptococcal strains to acquire multidrug resistance genes, underscoring the need for increased vigilance regarding the resistance traits and transmission risks of animal-derived streptococci.

Streptococcus orisratti  /  multidrug resistance  /  resistance mechanisms  /  ermB  /  optrA
Haowen TAI, Jiaming ZHANG, Han ZHENG, Yuqing LIU, Jingyi ZHANG, Huiqiang FU, Yifan ZHANG, Juan LI. Characterization of the first penicillin-resistant Streptococcus orisratti strain carrying both ermB and optrA in China[J]. Acta Microbiologica Sinica, 2026 , 66 (3) : 1311 -1325 . DOI: 10.13343/j.cnki.wsxb.20250787
Funding
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  • Public Health Talent Support Project of National Disease Control and Prevention Administration
  • Major Project of Guangzhou National Laboratory(GZNL2024A01025)
Appendix
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Year 2026 volume 66 Issue 3
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229
106
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Article Info
doi: 10.13343/j.cnki.wsxb.20250787
  • Receive Date：2025-10-21
  • Online Date：2026-03-12
  • Published：2026-03-04
Article Data
Affiliations
History
  • Received：2025-10-21
  • Accepted：2025-12-23
Funding
Public Health Talent Support Project of National Disease Control and Prevention Administration
Major Project of Guangzhou National Laboratory(GZNL2024A01025)
Affiliations
    1.National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China
    2.National Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China

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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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