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Probiotic characterization and whole genome sequencing of Limosilactobacillus reuteri CHF7-2 from Chahua chicken
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Le XU1, 2, Shiyu CHEN1, Shang WANG1, Zhixiang ZHANG1, Bin DONG1, Qiuye LIN3, *, Zhenhui CAO1, 2, *
Acta Microbiologica Sinica | 2025, 65(3) : 1197 - 1218
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Acta Microbiologica Sinica | 2025, 65(3): 1197-1218
• Research Article •
Probiotic characterization and whole genome sequencing of Limosilactobacillus reuteri CHF7-2 from Chahua chicken
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Le XU1, 2, Shiyu CHEN1, Shang WANG1, Zhixiang ZHANG1, Bin DONG1, Qiuye LIN3, *, Zhenhui CAO1, 2, *
Affiliations
  • 1 Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, China
  • 2 Key Laboratory of Animal Nutrition and Feed Science of Yunnan Province, Kunming, Yunnan, China
  • 3 College of Food Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, China
Published: 2025-03-04 doi: 10.13343/j.cnki.wsxb.20240642
Outline
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[Objective] To study the probiotic properties and safety of Limosilactobacillus reuteri CHF7-2 isolated from Chahua chicken, providing a theoretical basis for the development of this strain as a microecological feed additive. [Methods] In vitro assays were conducted to evaluate the adhension, enzyme production, antibacterial activity, and antioxidant activity of strain CHF7-2. PacBio Sequel II and Illumina NovaSeq 6000 were used for whole genome sequencing of strain CHF7-2. Bioinformatics tools and databases were then used for genome annotation to explore the probiotic mechanisms and safety of this strain at the molecular level. [Results] Strain CHF7-2 possessed significant probiotic properties and safety. It exhibited high surface hydrophobicity, self-coagulation, and antioxidant activity. The strain inhibited the growth of Escherichia coli K88, Staphylococcus aureus ATCC 49521, Salmonella gallinarum CICC 21510, and Salmonella choleraesuis CVCC 3383. Strain CHF7-2 produced protease and lipase, and it was safe for use in feed since it did not exhibit hemolytic activity. Whole genome sequencing revealed that the genome size of CHF7-2 was 2 116 761 bp, with the G+C content of 38.8%, encoding 2 067 genes. Additionally, the genome carried the biosynthetic gene cluster of the class III bacteriocin EnlA and multiple genes involved in the acid tolerance, bile salt tolerance, heat stress tolerance, cold stress tolerance, adhesion, antioxidation, and organic acid synthesis, with no virulence or antibiotic resistance genes detected. [Conclusion] L. reuteri CHF7-2 is a potential probiotic strain and a promising candidate for use as a microecological feed additive.

Limosilactobacillus reuteri  /  whole genome sequencing  /  probiotic properties  /  microecological feed additive
Le XU, Shiyu CHEN, Shang WANG, Zhixiang ZHANG, Bin DONG, Qiuye LIN, Zhenhui CAO. Probiotic characterization and whole genome sequencing of Limosilactobacillus reuteri CHF7-2 from Chahua chicken[J]. Acta Microbiologica Sinica, 2025 , 65 (3) : 1197 -1218 . DOI: 10.13343/j.cnki.wsxb.20240642
Funding
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  • Yunnan Provincial Middle-young Academic and Technical Leader Candidate(202305AC160040)
  • Young Top-notch Talent of Yunnan Xingdian Support Project for High Level Talents(YNWR-QNBJ-2018-137)
Appendix
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Year 2025 volume 65 Issue 3
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146
53
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Article Info
doi: 10.13343/j.cnki.wsxb.20240642
  • Receive Date：2024-10-16
  • Online Date：2026-02-10
  • Published：2025-03-04
Article Data
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History
  • Received：2024-10-16
  • Accepted：2024-11-25
Funding
Yunnan Provincial Middle-young Academic and Technical Leader Candidate(202305AC160040)
Young Top-notch Talent of Yunnan Xingdian Support Project for High Level Talents(YNWR-QNBJ-2018-137)
Affiliations
    1 Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, China
    2 Key Laboratory of Animal Nutrition and Feed Science of Yunnan Province, Kunming, Yunnan, China
    3 College of Food Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, China

Corresponding:

*E-mail: LIN Qiuye,
CAO Zhenhui,
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Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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