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Construction and biological characterization of Brucella S2Δbp26
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Shibo LIU1, 2, Xinyue TANG1, 3, Xiaoqian ZHANG1, Yinghui ZHANG1, Jihui JIN1, Weifeng SUN1, Xiaowei PENG1, *, Junping LI1, *
Acta Microbiologica Sinica | 2025, 65(3) : 1137 - 1147
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Acta Microbiologica Sinica | 2025, 65(3): 1137-1147
• Research Article •
Construction and biological characterization of Brucella S2Δbp26
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Shibo LIU1, 2, Xinyue TANG1, 3, Xiaoqian ZHANG1, Yinghui ZHANG1, Jihui JIN1, Weifeng SUN1, Xiaowei PENG1, *, Junping LI1, *
Affiliations
  • 1 National Reference Laboratory for Animal Brucellosis, China Institute of Veterinary Drug Control, Beijing, China
  • 2 College of Animal Medicine, Jilin Agricultural University, Changchun, Jilin, China
  • 3 Animal Husbandry & Veterinary Station of Beijing SanYuan Group, Beijing, China
Published: 2025-03-04 doi: 10.13343/j.cnki.wsxb.20240645
Outline
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[Objective] To develop a safe, effective, and identifiable new vaccine. [Methods] The gene deletion strain S2Δbp26 was constructed by homologous recombination with S2 as the parental strain. The stability, safety, and efficacy of S2Δbp26 were then evaluated. [Results] The phenotype and genotype of S2Δbp26 did not change after 20 successive passages invitro. The experiments in mice and guinea pigs showed that there was no difference in biosafety between S2Δbp26 and S2. The bacterial load was less than 2×105 CFU per gram of spleen in guinea pigs inoculated with S2Δbp26 at different passages and S2, which suggested that the virulence of the mutant strain was attenuated. The mice inoculated with S2ΔBP26 had a shorter 50% recovery time than those inoculated with S2. Meanwhile, the mice immunized with S2Δbp26 could successfully resist the challenge with M28 wild-type strain at a dose of 2×105 CFU/mouse. [Conclusion] S2Δbp26 was successfully constructed, with excellent safety and immunoprotective capacity, which provided technical reserves for the development of identifiable vaccines for brucellosis.

Brucella  /  vaccine  /  S2Δbp26  /  biological characterization
Shibo LIU, Xinyue TANG, Xiaoqian ZHANG, Yinghui ZHANG, Jihui JIN, Weifeng SUN, Xiaowei PENG, Junping LI. Construction and biological characterization of Brucella S2Δbp26[J]. Acta Microbiologica Sinica, 2025 , 65 (3) : 1137 -1147 . DOI: 10.13343/j.cnki.wsxb.20240645
Funding
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  • National Key Research and Development Program of China(2022YFD1800600)
  • National Key Research and Development Program of China(2022YFD1800601)
Appendix
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Year 2025 volume 65 Issue 3
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65
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Article Info
doi: 10.13343/j.cnki.wsxb.20240645
  • Receive Date：2024-10-16
  • Online Date：2026-02-10
  • Published：2025-03-04
Article Data
Affiliations
History
  • Received：2024-10-16
  • Accepted：2024-11-20
Funding
National Key Research and Development Program of China(2022YFD1800600)
National Key Research and Development Program of China(2022YFD1800601)
Affiliations
    1 National Reference Laboratory for Animal Brucellosis, China Institute of Veterinary Drug Control, Beijing, China
    2 College of Animal Medicine, Jilin Agricultural University, Changchun, Jilin, China
    3 Animal Husbandry & Veterinary Station of Beijing SanYuan Group, Beijing, China

Corresponding:

*E-mail: LI Junping,
PENG Xiaowei,
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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