PDF
Full
Outline
[Objective] This study investigates the effects of various diets on the structure and diversity of the fungal community in the tobacco beetle Lasioderma serricorne, aiming to provide a theoretical basis for developing green control strategies for stored-product pests through microbial regulation. [Methods] The PacBio SMRT platform was used for full-length internal transcribed spacer (ITS) amplicon sequencing, on the basis of which the community structure characteristics of fungi in L. serricorne were compared among three groups: artificial feed (SL), tobacco domestication (YC), and wild environment (WF). Additionally, culturable fungi were isolated via the culture method, and the tissue expression pattern of the core symbiotic fungus Symbiotaphrina kochii was localized by RT-qPCR. [Results] SL, YC, and WF groups contained 35, 32, and 15 operational taxonomic units (OTUs), respectively. The core OTUs shared by the three groups accounted for 31.43%, 34.38%, and 73.33% in SL, YC, and WF groups, respectively. The Sobs index of the SL group was 29.00±1.13, which was higher than those of the YC group (16.17±2.30) and WF group (12.33±1.33) (P<0.001). Symbiotaphrina was the core functional group shared by the three groups, and its relative abundance was more than 81.000 0% in all the three groups. Aspergillus and Xeromyces were the characteristic genera of the SL and YC groups, while Symbiotaphrinabuchneri and Symbiotaphrina microtheca formed the evolutionary clades specific to the YC and WF groups. Eight Ascomycota strains were isolated via the culture method, belonging to three genus: Symbiotaphrina (three strains), Talaromyces (three strains), and Penicillium (two strains). Tissue-specific expression analysis confirmed the higher expression level of S. kochii in mycetocytes (10.42±1.03) than in the fat body (0.74±0.08) and midgut (0.31±0.01) (P<0.001), validating its intracellular colonization. [Conclusion] This study for the first time reveals that diets regulate the fungal community assembly in L. serricorne through a “nutrient-microbiota” interaction network and demonstrates the pivotal role of Symbiotaphrina in adaptive evolution of the host. These findings establish a theoretical foundation and provide critical targets for developing precision pest control technologies based on targeted modulation of microbial interaction networks.
PDF
149
55
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
