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Construction, expression, and characterization of secretory IgA antibody against foot-and-mouth disease virus
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Feng LIU1, 2, Kun LI1, 2, Xueqing MA1, 2, Yifang OUYANG1, 2, Pu SUN1, 2, Xingze ZHANG1, 2, Yimei CAO1, 2, Xingwen BAI1, 2, Hong YUAN1, 2, Fengjuan LI1, 2, Zaixin LIU1, 2, Zengjun LU1, 2, Pinghua LI1, 2
Acta Microbiologica Sinica | 2025, 65(9) : 3992 - 4000
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Acta Microbiologica Sinica | 2025, 65(9): 3992-4000
• Research Article •
Construction, expression, and characterization of secretory IgA antibody against foot-and-mouth disease virus
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Feng LIU1, 2, Kun LI1, 2, Xueqing MA1, 2, Yifang OUYANG1, 2, Pu SUN1, 2, Xingze ZHANG1, 2, Yimei CAO1, 2, Xingwen BAI1, 2, Hong YUAN1, 2, Fengjuan LI1, 2, Zaixin LIU1, 2, Zengjun LU1, 2, Pinghua LI1, 2
Affiliations
  • 1 State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China
  • 2 Gansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou, Gansu, China
Published: 2025-09-04 doi: 10.13343/j.cnki.wsxb.20250125
Outline
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[Objective] To prepare secretory IgA (sIgA) antibody against foot-and-mouth disease virus (FMDV) and provide a new idea and basis for the comprehensive prevention and control of foot-and-mouth disease (FMD). [Methods] We constructed an eukaryotic expression plasmid harboring the heavy chain of IgA antibody by replacing the constant region gene of heavy chain of IgG with the codon-optimized corresponding region of IgA based on POA-8, a previously screened IgG neutralizing antibody capable of neutralizing both type O and type A FMDV. At the same time, the eukaryotic expression plasmids harboring the J chain and secretory component (SC) of pigs as well as the eukaryotic expression plasmid containing heavy chain, light chain, and J chain genes were constructed respectively. CHO-S cells were transiently co-transfected with 4 plasmids (heavy, light, J, and SC) or 2 plasmids (heavy+light+J and SC) for assembling and expression of sIgA antibody. sIgA antibody was purified and identified by SDS-PAGE and Western blotting. The binding activity and neutralizing activity of sIgA antibody against FMDV were evaluated by indirect ELISA and virus neutralization assay. [Results] sIgA antibody against FMDV could be successfully assembled and expressed after transfection of CHO-S cells with 4 or 2 plasmids. The binding and neutralizing abilities of sIgA against type O and type A FMDV were stronger than those of IgG. [Conclusion] This study established an efficient expression system for sIgA against FMDV, laying a foundation for the development of mucosal vaccines and antiviral drugs against FMDV in the future.

foot-and-mouth disease virus  /  secreted IgA antibody  /  construction and expression  /  characterization
Feng LIU, Kun LI, Xueqing MA, Yifang OUYANG, Pu SUN, Xingze ZHANG, Yimei CAO, Xingwen BAI, Hong YUAN, Fengjuan LI, Zaixin LIU, Zengjun LU, Pinghua LI. Construction, expression, and characterization of secretory IgA antibody against foot-and-mouth disease virus[J]. Acta Microbiologica Sinica, 2025 , 65 (9) : 3992 -4000 . DOI: 10.13343/j.cnki.wsxb.20250125
Funding
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  • Innovation Program of Chinese Academy of Agricultural Sciences(CAAS-CSLPDCP-202402)
  • National Key Research and Development Program of China(2021YFD1800300)
Appendix
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Year 2025 volume 65 Issue 9
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Article Info
doi: 10.13343/j.cnki.wsxb.20250125
  • Receive Date：2025-02-21
  • Online Date：2026-02-07
  • Published：2025-09-04
Article Data
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History
  • Received：2025-02-21
  • Accepted：2025-04-09
Funding
Innovation Program of Chinese Academy of Agricultural Sciences(CAAS-CSLPDCP-202402)
National Key Research and Development Program of China(2021YFD1800300)
Affiliations
    1 State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China
    2 Gansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou, Gansu, China

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多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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