PDF
Full
Outline
[Objective] To investigate the role of lmo2300 in oxidative stress resistance and infection of Listeria monocytogenes. [Methods] With L. monocytogenes 1/2 a serotype EGD-e as the parent strain, the lmo2300-deleted strain and complementary strain were constructed by homologous recombination. The growth, motility, oxidative stress resistance, minimum inhibitory concentration and reductase activity of Δlmo2300 were evaluated. The adhesion, invasion, proliferation, and intercellular migration abilities of the Δlmo2300 strain was assessed using cell models. The transcriptional levels of thioredoxin-related genes and major virulence genes in Δlmo2300 were detected with RT-qPCR. [Results] PCR identification and DNA sequencing confirmed the successful construction of Δlmo2300 and CΔlmo2300. Deletion of lmo2300 did not affect the bacterial growth, motility, antibiotic susceptibility, adhesion, invasion or intracellular proliferation. However, the Lmo2300 protein possesses reductase activity, and the transcriptional levels of the thioredoxin gene lmo1903 and grx are significantly upregulated in the Δlmo2300 mutant. And markedly improved resistance to oxidative substances, including H2O2, diamide, CdCl2, and MnSO4 compared with the wild type. The Δlmo2300 mutation significantly enhanced intercellular migration ability, accompanied by an approximately 8-fold upregulation of the actA gene transcription, which is consistent with the enhanced intercellular migration ability. [Conclusion] This study demonstrates that lmo2300 plays a critical role in modulating the oxidative stress resistance and intercellular migration of L. monocytogenes, providing novel insights into the infection biology and adaptive responses of L. monocytogenes to host-derived oxidative challenges.
PDF
151
60
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
