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CO2-fixing microbial strains in the grassland soil of the Loess Plateau: characteristics and CO2 fixation conditions
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Fadan LEI1, Qian HUANG1, Xiaoyu LU1, Yuqing CAO1, Bin WEI1, Jiayi XU1, Fang CHEN1, Baorong WANG2, Yimei HUANG1, *
Acta Microbiologica Sinica | 2025, 65(6) : 2642 - 2654
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Acta Microbiologica Sinica | 2025, 65(6): 2642-2654
• Research Article •
CO2-fixing microbial strains in the grassland soil of the Loess Plateau: characteristics and CO2 fixation conditions
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Fadan LEI1, Qian HUANG1, Xiaoyu LU1, Yuqing CAO1, Bin WEI1, Jiayi XU1, Fang CHEN1, Baorong WANG2, Yimei HUANG1, *
Affiliations
  • 1.Key Laboratory of Low-carbon Green Agriculture in Northwestern China, Ministry of Agriculture and Rural Affairs, College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi, China
  • 2.College of Grassland Agriculture, Northwest A&F University, Yangling, Shaanxi, China
Published: 2025-06-04 doi: 10.13343/j.cnki.wsxb.20250116
Outline
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[Objective] To screen CO2-fixing microbial strains in grassland soil, clarify their physiological and biochemical characteristics, and determine their optimal CO2 fixation conditions, providing a theoretical basis for understanding the mechanisms of soil CO2 fixation and enhancing grassland soil carbon sink. [Methods] The carbon-free solid medium and the dilution plating method were employed to isolate CO2-fixing microbial strains from grassland soil in the Loess Plateau. The strains were identified via 16S rRNA gene sequencing. Physiological and biochemical characteristics of the strains were determined by Gram staining and starch hydrolysis, indole production, methyl red, and lactic acid fermentation tests. Culture experiments with carbon-free liquid medium and sterilized soil were carried out to assess the CO2 fixation efficiency under varying temperatures (20-40 ℃), soil moisture levels (3.00%-27.00%), and the presence of electron donors (NaNO2 and Na2S). [Results] Nine bacterial strains with effective CO2 fixation were isolated, belonging to Bacillus, Streptomyces, Sinorhizobium, Agrobacterium, Enterobacter, Brevundimonas, and Prolinoborus. Among them, strains A4 (Agrobacterium) and A7 (Brevundimonas) exhibited the highest CO2 fixation efficiency (P<0.05), both being Gram-positive. Optimal conditions for CO2 fixation of the nine strains were 25-30 ℃ and soil moisture of 3.00%, 15.00%, or 27.00% (P<0.05). Electron donor supplementation enhanced CO2 fixation efficiency, and the enhancement effect followed the order: single addition of NaNO2>single addition of Na2S>co-addition of NaNO2+Na2S (P<0.05). [Conclusion] This study identified the dominant CO2-fixing microbial strains and the conditions suitable for CO2 fixation, providing a theoretical basis and technical support for enhancing the carbon sink of the terrestrial ecosystem on the Loess Plateau and mitigating the greenhouse effect.

Loess Plateau grassland  /  CO2-fixing microbial strains  /  CO2 fixation conditions  /  CO2 fixation efficiency  /  electron donor
Fadan LEI, Qian HUANG, Xiaoyu LU, Yuqing CAO, Bin WEI, Jiayi XU, Fang CHEN, Baorong WANG, Yimei HUANG. CO2-fixing microbial strains in the grassland soil of the Loess Plateau: characteristics and CO2 fixation conditions[J]. Acta Microbiologica Sinica, 2025 , 65 (6) : 2642 -2654 . DOI: 10.13343/j.cnki.wsxb.20250116
Funding
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  • National Natural Science Foundation of China(42477372)
  • College Students’ Innovative Entrepreneurial Training Program of Northwest A&F University(XN2025007089)
Appendix
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Year 2025 volume 65 Issue 6
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151
63
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Article Info
doi: 10.13343/j.cnki.wsxb.20250116
  • Receive Date：2025-02-19
  • Online Date：2026-02-07
  • Published：2025-06-04
Article Data
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History
  • Received：2025-02-19
  • Accepted：2025-04-08
Funding
National Natural Science Foundation of China(42477372)
College Students’ Innovative Entrepreneurial Training Program of Northwest A&F University(XN2025007089)
Affiliations
    1.Key Laboratory of Low-carbon Green Agriculture in Northwestern China, Ministry of Agriculture and Rural Affairs, College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi, China
    2.College of Grassland Agriculture, Northwest A&F University, Yangling, Shaanxi, China

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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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