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Advances in resource exploration and metabolic mechanism of denitrifying phosphorus-accumulating organisms in wastewater from a single-cell perspective
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Pengcheng SUN1, 2, Huihui PAN2, Yushu JING4, Wenxiang XIA1, Yishang REN2, Xiaoyan JING2, 3, *
Acta Microbiologica Sinica | 2025, 65(4) : 1650 - 1666
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Acta Microbiologica Sinica | 2025, 65(4): 1650-1666
• New technologies and methods for microbial resources •
Advances in resource exploration and metabolic mechanism of denitrifying phosphorus-accumulating organisms in wastewater from a single-cell perspective
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Pengcheng SUN1, 2, Huihui PAN2, Yushu JING4, Wenxiang XIA1, Yishang REN2, Xiaoyan JING2, 3, *
Affiliations
  • 1.School of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao, Shandong, China
  • 2.Single Cell Center, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, China
  • 3.College of Biological Engineering, Qingdao University of Science and Technology, Qingdao, Shandong, China
  • 4.Qingdao Water Group Environmental Energy Co. , Ltd. , Qingdao, Shandong, China
Published: 2025-04-04 doi: 10.13343/j.cnki.wsxb.20240650
Outline
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Amidst the escalating issues of water eutrophication and water resource scarcity, the development of high-efficiency wastewater treatment technologies has become increasingly imperative. Traditional nitrogen and phosphorus removal processes face challenges in achieving efficient simultaneous elimination of nitrogen and phosphorus due to the disparities in sludge age and the competition for carbon sources among microorganisms. Denitrifying phosphorus-accumulating organisms (DPAOs) possess the capability to remove both nitrogen and phosphorus, demonstrating significant potential in wastewater treatment. However, population-level studies often overlook cellular heterogeneity, leading to an inadequate understanding of the nitrogen and phosphorus removal mechanisms of DPAOs. Moreover, the “traditional culture-first, screen-second” method yields a limited number of efficient DPAO strains, the stability and adaptability of which face challenges in actual wastewater treatment environments. Single-cell analysis technologies provide new perspectives for a deeper understanding of microbial ecological niches and metabolic mechanisms. Coupling non-destructive single-cell phenotypic identification technologies, such as single-cell Raman spectroscopy (SCRS), with the culture method paves new avenues for the exploration of DPAO strains. This review summarizes the research status and progress in the exploration of DPAO strain resources and their metabolic mechanisms, focusing on the potential of single-cell technologies in revealing the mechanisms of nitrogen and phosphorus removal by DPAOs and in the exploration of DPAO resources. The aim is to provide a new theoretical foundation and technical support for the research and application of DPAOs, thereby promoting the development of efficient wastewater treatment technologies.

single-cell technology  /  denitrifying phosphorus-accumulating organisms  /  Raman spectrum  /  microbial resource mining  /  metabolic mechanism
Pengcheng SUN, Huihui PAN, Yushu JING, Wenxiang XIA, Yishang REN, Xiaoyan JING. Advances in resource exploration and metabolic mechanism of denitrifying phosphorus-accumulating organisms in wastewater from a single-cell perspective[J]. Acta Microbiologica Sinica, 2025 , 65 (4) : 1650 -1666 . DOI: 10.13343/j.cnki.wsxb.20240650
Funding
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  • National Natural Science Foundation of China(32270109)
Appendix
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Year 2025 volume 65 Issue 4
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136
48
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Article Info
doi: 10.13343/j.cnki.wsxb.20240650
  • Receive Date：2024-10-21
  • Online Date：2026-02-06
  • Published：2025-04-04
Article Data
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History
  • Received：2024-10-21
  • Accepted：2024-12-09
Funding
National Natural Science Foundation of China(32270109)
Affiliations
    1.School of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao, Shandong, China
    2.Single Cell Center, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, China
    3.College of Biological Engineering, Qingdao University of Science and Technology, Qingdao, Shandong, China
    4.Qingdao Water Group Environmental Energy Co. , Ltd. , Qingdao, Shandong, China

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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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