PDF
Full
Outline
[Objective] This study isolated and identified an algicidal bacterium from the East China Sea near Wenzhou and investigated its algicidal characteristics and mechanisms, aiming to contribute a solid scientific basis to the microbial control against red tides. [Methods] The strain was identified by morphological observation, physiological and biochemical tests, and 16S rRNA gene sequence analysis. The algicidal characteristics such as algicidal activity, influences of environmental factors on the algicidal activity, and algicidal specificity were assessed. The algicidal mechanism was explored by electron microscopy, measurements of photosynthetic parameters, and determination of reactive oxygen species (ROS) and malondialdehyde levels and antioxidant enzyme activities. [Results] The algicidal strain J75 was identified as Pseudoalteromonas sp. The algicidal rate of strain J75 against Skeletonemacostatum reached 95.97% within 36 h. Strain J75 induced lysis of algal cells indirectly by secreting extracellular algicidal substances, maintaining the algicidal activity across broad ranges of temperatures (-20 °C to 80 °C) and pH levels (5.0 to 9.0). In addition, strain J75 demonstrated algicidal activities against other harmful microalgae, including Kareniamikimotoi, Prorocentrumminimum, and Phaeocystisglobosa. Under the stress of J75 cell-free supernatant, the algal cells showed morphological structure damage, a significant decrease in the photosynthetic activity, elevations in the levels of ROS, membrane lipid peroxidation, activities of superoxide dismutase, catalase, and peroxidase, and the level of glutathione. These results indicated that strain J75 caused oxidative damage to algal cells, ultimately leading to algal death. [Conclusion] The marine algicidal bacterium Pseudoalteromonas sp. J75, which holds significant potential for the control of red tides, demonstrates its efficacy by secreting algicidal compounds that inhibit the growth of diverse red tide microalgae. The exploration of the algicidal characteristics and mechanisms of this strain provides a theoretical basis for advancing red tide management strategies.
PDF
144
60
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
