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[Objective] Chitin lyases are the key enzymes for bacteria to degrade chitin. Pseudoalteromonas arabiensis N1230-9 possesses five chitin lyase-encoding genes (woc28159, woc28160, woc28161, woc27404, and woc27232). Functional identification of these five genes is crucial for determining the ability of strain to degrade chitin. [Methods] The distribution patterns of five chitin lyases from strain N1230-9 in 44 model strains of Pseudomonas were analyzed by bioinformatics methods. The transcription levels of five chitin lyase-encoding genes of strain N1230-9 cultured in the medium with chitin as the sole carbon source were analyzed by RT-qPCR. The mutants with deletion of chitin lyase-encoding genes were constructed by homologous recombination, and their abilities to degrade chitin were evaluated. [Results] Thirty-three Pseudoalteromonas strains possessed chitin lyase homologous proteins of strain N1230-9. Chitin significantly upregulated the transcription levels of woc28159, woc28160, woc28161, and woc27404, and the deletion of each of the four genes weakened the chitin-degradation ability. The transcription level of woc27232 was not affected by chitin, and the deletion of this gene did not affect the chitin-degradation ability of the strain. [Conclusion] WOC28159 and WOC28161 are necessary for the degradation of chitin by strain N1230-9. WOC28160 and WOC27404 endow strain N1230-9 with efficient chitin-degrading ability, while WOC27232 does not participate in the chitin degradation process.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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