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Characteristics of a strain of foot-and-mouth disease virus isolated from bovine oropharyngeal fluid
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Mengting CAI1, Hong YUAN1, 2, Lihao WANG3, Jingchun YANG1, Tao WANG1, Huifang BAO1, 2, Yuxuan YANG1, 2, Pu SUN1, 2, Kun LI1, 2, 4, Shulun HUANG1, 2, Haiyang GUO1, 2, Pinghua LI1, 2, Zengjun LU1, 2, 4, Jijun HE1, 2, 4, Xingwen BAI1, 2, 4
Acta Microbiologica Sinica | 2025, 65(7) : 2938 - 2947
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Acta Microbiologica Sinica | 2025, 65(7): 2938-2947
• Research Article •
Characteristics of a strain of foot-and-mouth disease virus isolated from bovine oropharyngeal fluid
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Mengting CAI1, Hong YUAN1, 2, Lihao WANG3, Jingchun YANG1, Tao WANG1, Huifang BAO1, 2, Yuxuan YANG1, 2, Pu SUN1, 2, Kun LI1, 2, 4, Shulun HUANG1, 2, Haiyang GUO1, 2, Pinghua LI1, 2, Zengjun LU1, 2, 4, Jijun HE1, 2, 4, Xingwen BAI1, 2, 4
Affiliations
  • 1.State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China
  • 2.World Organization for Animal Health (WOAH)/National Foot and Mouth Disease Reference Laboratory, Lanzhou, Gansu, China
  • 3.College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China
  • 4.Gansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou, Gansu, China
Published: 2025-07-04 doi: 10.13343/j.cnki.wsxb.20240805
Outline
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[Objective] To isolate and identify a strain of foot-and-mouth disease virus (FMDV) from bovine oropharyngeal fluid (OPF) and subsequently analyze the amino acid mutations in antigenic proteins and the proliferative properties of the isolated virus. [Methods] RT-PCR and sequencing were performed to identify the FMDV-positive OPF samples. The FMDV strain was isolated from bovine OPF samples by cell inoculation and identified by indirect immunofluorescence assay. The whole genome sequence of the strain was obtained by fragment amplification and sequencing. The amino acid sequences were compared to reveal the amino acid mutations in the antigenic proteins of the strain. The proliferative properties of the isolated FMDV strain were analyzed by the plaque forming assay and one-step growth curve. [Results] A FMDV strain O/FMDV/OP/2022/B was successfully isolated from bovine OPF, belonging to serotype O and Ind-2001 lineage. Amino acid mutations were identified in the antigenic proteins VP1, VP2, and VP3 of O/FMDV/OP/2022/B. In addition, the plaque of O/FMDV/OP/2022/B in BHK-21 cells was smaller than that of the vaccine strain O/FMDV/HN/93. The one-step growth curves showed that O/FMDV/OP/2022/B had significantly lower replication titer than O/FMDV/HN/93 in BHK-21 cells. [Conclusion] A strain of FMDV was isolated from bovine OPF, and it displayed amino acid mutations in antigenic proteins and had lower proliferative capacity than the vaccine strain O/FMDV/HN/93. This study contributes to a better understanding of the mechanism of persistent FMDV infection in the bovine oropharynx and provides a reference for the prevention and control of FMD.

foot-and-mouth disease virus  /  persistent infection  /  amino acid mutation  /  viral proliferation
Mengting CAI, Hong YUAN, Lihao WANG, Jingchun YANG, Tao WANG, Huifang BAO, Yuxuan YANG, Pu SUN, Kun LI, Shulun HUANG, Haiyang GUO, Pinghua LI, Zengjun LU, Jijun HE, Xingwen BAI. Characteristics of a strain of foot-and-mouth disease virus isolated from bovine oropharyngeal fluid[J]. Acta Microbiologica Sinica, 2025 , 65 (7) : 2938 -2947 . DOI: 10.13343/j.cnki.wsxb.20240805
Funding
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  • National Key Research and Development Program of China(2021YFD1800300)
  • Science and Technology Plan of Gansu Province(23JRRA1515)
  • Major Science and Technology Project of Gansu Province(21ZD3NA001)
  • Major Science and Technology Project of Gansu Province(22ZD6NA001)
  • Major Science and Technology Project of Gansu Province(22ZD6NA012)
  • Project of National Center of Technology Innovation for Pigs(NCTIP-XD/C03)
Appendix
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Year 2025 volume 65 Issue 7
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180
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Article Info
doi: 10.13343/j.cnki.wsxb.20240805
  • Receive Date：2024-12-12
  • Online Date：2026-02-06
  • Published：2025-07-04
Article Data
Affiliations
History
  • Received：2024-12-12
  • Accepted：2025-02-07
Funding
National Key Research and Development Program of China(2021YFD1800300)
Science and Technology Plan of Gansu Province(23JRRA1515)
Major Science and Technology Project of Gansu Province(21ZD3NA001)
Major Science and Technology Project of Gansu Province(22ZD6NA001)
Major Science and Technology Project of Gansu Province(22ZD6NA012)
Project of National Center of Technology Innovation for Pigs(NCTIP-XD/C03)
Affiliations
    1.State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China
    2.World Organization for Animal Health (WOAH)/National Foot and Mouth Disease Reference Laboratory, Lanzhou, Gansu, China
    3.College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China
    4.Gansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou, Gansu, China

Corresponding:

*E-mail: HE Jijun,
BAI Xingwen,
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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