PDF
Full
Outline
[Objective] To extract and purify extracellular polysaccharides (EPS) from Sphingomonas echinoides 1K04342 and investigate the ameliorating effects and mechanisms of the purified EPS on glucose and lipid metabolism disorders induced by oleic acid (OA) in human hepatoma cells (HepG2). [Methods] A response surface methodology was employed to optimize the ultrasound power, ultrasound time, and incubation time for maximizing the EPS yield of S. echinoides 1K04342 from ultrasound-assisted extraction. The crude EPS underwent deproteinization and was further purified through a DEAE-Sepharose Fast Flow anion exchange column. The effects of the purified EPS on the proliferation, glucose uptake, and α-glucosidase inhibition and pancreatic lipase inhibition rates in HepG2 cells were evaluated. The most effective EPS component for mitigating OA-induced glucose and lipid metabolism disorders in IR-HepG2 cells was selected for in-depth physicochemical and bioactivity analyses. [Results] The optimal EPS extraction parameters were as follows: ultrasound power at 59.39 W, extraction for 42.08 s, and incubation for 9.24 h, under which the estimated yield of EPS reached 2.13 g/L. Under the adjusted parameters (60 W, 42 s, and 9.2 h), the EPS yield was 2.10 g/L, which was statistically consistent with the theoretical value (2.13 g/L). The optimal component for ameliorating glucose and lipid metabolism disorders in IR-HepG2 cells was EPS-2, which showed the total sugar content of 74.3%, with a monosaccharide profile comprising fucose, rhamnose, arabinose, galactose, glucose, and mannose. EPS-2 alleviated OA-induced glucose and lipid metabolic disorders in IR-HepG2 cells. EPS-2 reversed OA-induced decreases in cell viability. EPS-2 may activate the IRS-1/PI3K/AKT pathway, up-regulating the protein levels of GSK3β and FoxO1 and down-regulating the mRNA and protein levels of PEPCK and G6Pase, thereby mitigating glucose metabolism disorders. EPS-2 may regulate OA-induced lipid metabolism disorders by activating the AMPK/ACC1/SREBP-1C pathway, lowering the triacylglycerol (TG), total cholesterol (TC), and low-density lipoprotein (LDL) levels, and increasing the high-density lipoprotein (HDL) level. [Conclusion] EPS-2 derived from S. echinoides 1K04342 could effectively ameliorate glucose and lipid metabolism disorders in IR-HepG2 cells.
PDF
133
48
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
