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Discovery and characterization of a new enzyme for hydrolyzing polyethylene terephthalate (PET)
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Wei ZHAO1, 3, Haizhen BI2, 3, Yew Maxine3, Huanhuan ZHAI3, Cuiying ZHANG1, Leilei ZHU3
Acta Microbiologica Sinica | 2025, 65(7) : 3126 - 3135
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Acta Microbiologica Sinica | 2025, 65(7): 3126-3135
• Research Article •
Discovery and characterization of a new enzyme for hydrolyzing polyethylene terephthalate (PET)
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Wei ZHAO1, 3, Haizhen BI2, 3, Yew Maxine3, Huanhuan ZHAI3, Cuiying ZHANG1, Leilei ZHU3
Affiliations
  • 1.School of Biological Engineering, Tianjin University of Science & Technology, Tianjin, China
  • 2.Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin, China
  • 3.State Key Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China
Published: 2025-07-04 doi: 10.13343/j.cnki.wsxb.20250014
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[Objective] The enzymatic depolymerization of polyethylene terephthalate (PET) involves the hydrolysis of PET catalyzed by PET hydrolases, leading to the production of terephthalic acid and ethylene glycol. In this study, we identified a new PET hydrolase through gene mining, with the goal of providing enzymes for efficient depolymerization of waste PET. [Methods] A previously uncharacterized functional enzyme, named as AePETase, was identified through gene mining. This enzyme demonstrated the ability to hydrolyze amorphous PET films. Subsequently, heterologous expression, purification, and enzymatic characterization were performed for AePETase. [Results] AePETase showed a Tm value of (53.0±0.7) ℃, and an optimal reaction temperature of 45 ℃. The optimal buffer was Na2HPO4-NaH2PO4 (100 mmol/L, pH 8.0), and the optimal enzyme concentration was 1 µmol/L. After 72 h, the hydrolysis of amorphous PET films by AePETase resulted in 13.4-fold more products than that by the reported IsPETase. [Conclusion] AePETase demonstrates significant hydrolytic activity on PET and represents a promising new enzyme for hydrolyzing PET.

polyethylene terephthalate (PET) hydrolase  /  gene mining  /  AePETase
Wei ZHAO, Haizhen BI, Yew Maxine, Huanhuan ZHAI, Cuiying ZHANG, Leilei ZHU. Discovery and characterization of a new enzyme for hydrolyzing polyethylene terephthalate (PET)[J]. Acta Microbiologica Sinica, 2025 , 65 (7) : 3126 -3135 . DOI: 10.13343/j.cnki.wsxb.20250014
Funding
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  • National Key Research and Development Program of China(2023YFC3905000)
Appendix
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Year 2025 volume 65 Issue 7
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Article Info
doi: 10.13343/j.cnki.wsxb.20250014
  • Receive Date：2025-01-06
  • Online Date：2026-02-06
  • Published：2025-07-04
Article Data
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History
  • Received：2025-01-06
  • Accepted：2025-03-04
Funding
National Key Research and Development Program of China(2023YFC3905000)
Affiliations
    1.School of Biological Engineering, Tianjin University of Science & Technology, Tianjin, China
    2.Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin, China
    3.State Key Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China

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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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