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The glycosyltransferaseWekO is involved in the infection and pathogenicity of avian pathogenic Escherichia coli
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Yujie GAO1, Jimian YU2, Xiaohui ZHANG1, Wenxin WANG1, Tongxin GE1, Ruofei SONG1, Haonan XU1, Dandan FU1, Houhui SONG1, Changyong CHENG1, *, Yue HAN1, *
Acta Microbiologica Sinica | 2025, 65(8) : 3630 - 3642
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Acta Microbiologica Sinica | 2025, 65(8): 3630-3642
• Research Article •
The glycosyltransferaseWekO is involved in the infection and pathogenicity of avian pathogenic Escherichia coli
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Yujie GAO1, Jimian YU2, Xiaohui ZHANG1, Wenxin WANG1, Tongxin GE1, Ruofei SONG1, Haonan XU1, Dandan FU1, Houhui SONG1, Changyong CHENG1, *, Yue HAN1, *
Affiliations
  • 1.Key Laboratory of Applied Biotechnology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Engineering Research Center for Animal Health Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, the Belt and Road International Joint Laboratory for One Health and Food Safety, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Veterinary Medicine of Zhejiang A&F University, Hangzhou, Zhejiang, China
  • 2.Ningbo College of Health Sciences, Ningbo, Zhejiang, China
Published: 2025-08-04 doi: 10.13343/j.cnki.wsxb.20250053
Outline
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[Objective] To study the effects of the glycosyltransferase WekO involved in O-antigen synthesis on the biological characteristics of avian pathogenic Escherichia coli (APEC). [Methods] The mutant strain ΔwekO of APEC O1 was constructed by Red homologous recombination, and the complementary strain CΔwekO was then constructed. The lipopolysaccharide (LPS) profile of each strain was identified by silver staining. Simultaneously, the growth rate and swimming motility were measured. The reactivity of each strain with rabbit anti-O1 serum was determined by Western blotting. The ability of ΔwekO to form biofilms was measured by the crystal violet staining method. DF-1 cells were used to evaluate the adhesion and invasion of ΔwekOin vitro. Subsequently, chicks were selected as an animal model to evaluate the pathogenicity of ΔwekO. [Results] The mutant strain ΔwekO and the complementary strain CΔwekO were constructed. The LPS profile of ΔwekO was incomplete compared with that of the wild-type strain. The mutant lacked O-antigen bands and showed no reactivity to anti-O1 serum. There was no significant difference in growth rate between different strains (P>0.05). However, the motility and biofilm formation capabilities of ΔwekO decreased (P<0.001). Additionally, ΔwekO demonstrated weakened adhesion to DF-1 cells (P<0.001) and demonstrated weakened invasion to DF-1 cells (P<0.01). Also, ΔwekO reduced pathogenicity to 7-day-old chicks (P<0.05). [Conclusion] The deletion of wekO results in impaired O-antigen synthesis, incomplete LPS profile, loss of flagellar and biofilm formation capabilities, and reduced pathogenicity of APEC. These findings are highly significant for improving the understanding of the role of glycosyltransferases involved in APEC O-antigen synthesis.

avian pathogenic Escherichia coli  /  lipopolysaccharide  /  O-antigen  /  glycosyltransferase  /  pathogenicity
Yujie GAO, Jimian YU, Xiaohui ZHANG, Wenxin WANG, Tongxin GE, Ruofei SONG, Haonan XU, Dandan FU, Houhui SONG, Changyong CHENG, Yue HAN. The glycosyltransferaseWekO is involved in the infection and pathogenicity of avian pathogenic Escherichia coli[J]. Acta Microbiologica Sinica, 2025 , 65 (8) : 3630 -3642 . DOI: 10.13343/j.cnki.wsxb.20250053
Funding
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  • National Key Research and Development Program of China(2023YFD1801000)
  • National Natural Science Foundation of China(32473033)
  • Natural Science Foundation of Zhejiang Province(LQ24C010005)
Appendix
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Year 2025 volume 65 Issue 8
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84
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Article Info
doi: 10.13343/j.cnki.wsxb.20250053
  • Receive Date：2025-01-17
  • Online Date：2026-02-06
  • Published：2025-08-04
Article Data
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History
  • Received：2025-01-17
  • Accepted：2025-02-19
Funding
National Key Research and Development Program of China(2023YFD1801000)
National Natural Science Foundation of China(32473033)
Natural Science Foundation of Zhejiang Province(LQ24C010005)
Affiliations
    1.Key Laboratory of Applied Biotechnology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Engineering Research Center for Animal Health Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, the Belt and Road International Joint Laboratory for One Health and Food Safety, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Veterinary Medicine of Zhejiang A&F University, Hangzhou, Zhejiang, China
    2.Ningbo College of Health Sciences, Ningbo, Zhejiang, China

Corresponding:

*E-mail: HAN Yue,
CHENG Changyong,
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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