Home Journals Articles Updates About
CN
image
收藏切换
An efficient chitinolytic bacterium Bacillus cereus BSF-CH1: isolation, identification, and application in puparium biotransformation of Hermetia illucens
收藏切换
PDF
Xiang YAO1, Qian LIU1, Shenghui WANG1, Jian YANG1, Caiwang PENG2, Wu CHEN1, Lijuan YIN1, Liangying DAI1, Yunsheng WANG1, *
Acta Microbiologica Sinica | 2025, 65(8) : 3721 - 3730
Less
收藏切换
Acta Microbiologica Sinica | 2025, 65(8): 3721-3730
• Research Article •
An efficient chitinolytic bacterium Bacillus cereus BSF-CH1: isolation, identification, and application in puparium biotransformation of Hermetia illucens
Full
Xiang YAO1, Qian LIU1, Shenghui WANG1, Jian YANG1, Caiwang PENG2, Wu CHEN1, Lijuan YIN1, Liangying DAI1, Yunsheng WANG1, *
Affiliations
  • 1.College of Plant Protection, Hunan Agricultural University, Changsha, Hunan, China
  • 2.College of Mechanical and Electrical Engineering, Hunan Agricultural University, Changsha, Hunan, China
Published: 2025-08-04 doi: 10.13343/j.cnki.wsxb.20250100
Outline
收藏切换

The puparium of Hermetia illucens is rich in chitin and protein, while efficient and environmentally friendly utilization methods remain to be developed. [Objective] To isolate chitinolytic bacteria from the puparium pile of H. illucens and explore their potential in puparium biotransformation. [Methods] Strains were isolated by the plate screening method and identified by 16S rRNA gene sequencing. The 3,5-dinitrosalicylic acid (DNS) method was employed to determine the chitinase activity. Whole genome sequencing by PacBio HiFi was conducted to elucidate the degradation mechanism. The application potential of the strain was explored by puparium fermentation experiments. [Results] Among the seven isolated strains, Bacillus cereus BSF-CH1 showed the highest chitinase activity, reaching a maximum chitinase activity of 0.48 U/mL on the second day of fermentation. The genome of BSF-CH1 contained three chitinase genes, seven chitin deacetylase genes, and four chitodextrinase genes. Puparium biotransformation experiments showed that BSF-CH1 could degrade 47.2% of puparium mass within 7 days, with degradation rates of 64.6% and 59.1% for chitin and protein, respectively. [Conclusion] This study reports an efficient chitinolytic bacterium isolated from the puparium of H. illucens, providing new insights into the puparium biotransformation and having important implications for promoting the sustainable development of the H. illucens industry.

chitinolytic bacteria  /  puparium of Hermetia illucens  /  Bacillus cereus  /  biotransformation  /  genome analysis
Xiang YAO, Qian LIU, Shenghui WANG, Jian YANG, Caiwang PENG, Wu CHEN, Lijuan YIN, Liangying DAI, Yunsheng WANG. An efficient chitinolytic bacterium Bacillus cereus BSF-CH1: isolation, identification, and application in puparium biotransformation of Hermetia illucens[J]. Acta Microbiologica Sinica, 2025 , 65 (8) : 3721 -3730 . DOI: 10.13343/j.cnki.wsxb.20250100
Funding
Less
  • General Program of the Natural Science Foundation of Hunan Province(2023JJ30310)
  • Scientific Research Project Funded by the Education Department of Hunan Province(22A0169)
Appendix
Less
Year 2025 volume 65 Issue 8
PDF
198
78
Cite this Article
BibTeX
Article Info
doi: 10.13343/j.cnki.wsxb.20250100
  • Receive Date：2025-02-13
  • Online Date：2026-02-06
  • Published：2025-08-04
Article Data
Affiliations
History
  • Received：2025-02-13
  • Accepted：2025-03-14
Funding
General Program of the Natural Science Foundation of Hunan Province(2023JJ30310)
Scientific Research Project Funded by the Education Department of Hunan Province(22A0169)
Affiliations
    1.College of Plant Protection, Hunan Agricultural University, Changsha, Hunan, China
    2.College of Mechanical and Electrical Engineering, Hunan Agricultural University, Changsha, Hunan, China

Corresponding:

References
Share
https://castjournals.cast.org.cn/joweb/wswxb/EN/10.13343/j.cnki.wsxb.20250100
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House