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A high-throughput pathway optimization strategy for enhancing biosynthesis of adipic acid
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Shuang LIU1, Yunying ZHAO1, 2, *, Shenghu ZHOU1, 2, Yu DENG1, 2, *
Acta Microbiologica Sinica | 2025, 65(8) : 3765 - 3781
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Acta Microbiologica Sinica | 2025, 65(8): 3765-3781
• Technology and Method •
A high-throughput pathway optimization strategy for enhancing biosynthesis of adipic acid
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Shuang LIU1, Yunying ZHAO1, 2, *, Shenghu ZHOU1, 2, Yu DENG1, 2, *
Affiliations
  • 1.Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, China
  • 2.School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, China
Published: 2025-08-04 doi: 10.13343/j.cnki.wsxb.20250067
Outline
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[Objective] Adipic acid is a key monomer for plastics such as nylon 66 and poly (butylene adipate-co-terephthalate) (PBAT), with a vast market potential. This study aims to explore the optimal expression levels of genes in the biosynthetic pathway of adipic acid. [Methods] We regulated the expression levels of genes in the adipic acid synthesis pathway by randomly combining gradient-strength constitutive promoters. The high-throughput screening based on an adipic acid biosensor was conducted to select the strain with the optimal combination. Subsequently, the fermentation media, carbon sources, metal ions, and precursor substance addition amounts were optimized. [Results] After screening, the optimal strain Escherichia coli MG1655 Δ8-D47 was obtained, with an adipic acid yield of 431.32 mg/L. After fermentation condition optimization, the yield of adipic acid in a shake flask reached 550.34 mg/L, which represented a 134% increase compared with that of the control strain Z1. [Conclusion] Metabolic pathway imbalance in microbial synthesis of adipic acid is the main factor limiting the increase in yield.

adipic acid  /  high-throughput screening  /  reverse adipate degradation pathway  /  promoter  /  metabolic balance
Shuang LIU, Yunying ZHAO, Shenghu ZHOU, Yu DENG. A high-throughput pathway optimization strategy for enhancing biosynthesis of adipic acid[J]. Acta Microbiologica Sinica, 2025 , 65 (8) : 3765 -3781 . DOI: 10.13343/j.cnki.wsxb.20250067
Funding
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  • National Key Research and Development Program of China(2022YFC2104600)
  • Distinguished Young Scholars of Jiangsu Province(BK20220089)
  • National Natural Science Foundation of China(22478156)
  • Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project(TSBICIP-KJGG-015)
Appendix
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Year 2025 volume 65 Issue 8
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Article Info
doi: 10.13343/j.cnki.wsxb.20250067
  • Receive Date：2025-01-23
  • Online Date：2026-02-06
  • Published：2025-08-04
Article Data
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History
  • Received：2025-01-23
  • Accepted：2025-03-10
Funding
National Key Research and Development Program of China(2022YFC2104600)
Distinguished Young Scholars of Jiangsu Province(BK20220089)
National Natural Science Foundation of China(22478156)
Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project(TSBICIP-KJGG-015)
Affiliations
    1.Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, China
    2.School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, China

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Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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