Home Journals Articles Updates About
CN
image
收藏切换
A multiplex PCR method for identification of virulent strains of Streptococcus suis serotype 4
收藏切换
PDF
Jinlu ZHU1, 2, 3, Zeren PENG1, 2, 3, Hongkun ZHUANG1, 2, 3, Jie SUN4, Zongfu WU1, 2, 3, 5, *
Acta Microbiologica Sinica | 2025, 65(8) : 3782 - 3793
Less
收藏切换
Acta Microbiologica Sinica | 2025, 65(8): 3782-3793
• Technology and Method •
A multiplex PCR method for identification of virulent strains of Streptococcus suis serotype 4
Full
Jinlu ZHU1, 2, 3, Zeren PENG1, 2, 3, Hongkun ZHUANG1, 2, 3, Jie SUN4, Zongfu WU1, 2, 3, 5, *
Affiliations
  • 1.College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, China
  • 2.Key Lab of Animal Bacteriology, Ministry of Agriculture and Rural Affairs, Nanjing, Jiangsu, China
  • 3.WOAH Reference Lab for Swine Streptococcosis, Nanjing, Jiangsu, China
  • 4.Animal and Plant Inspection and Quarantine Technology Centre of Shenzhen Customs, Shenzhen, Guangdong, China
  • 5.Guangdong Provincial Key Laboratory of Research on the Technology of Pig-breeding and Pig-disease Prevention, Guangdong Haid Institute of Animal Husbandry & Veterinary, Guangzhou, Guangdong, China
Published: 2025-08-04 doi: 10.13343/j.cnki.wsxb.20250119
Outline
收藏切换

Streptococcus suis is a major pathogen in pigs and also a zoonotic pathogen. This bacterium has numerous serotypes, among which S. suis serotype 4 (SS4) is known to infect humans and poses a threat to public health due to its potential high pathogenicity. [Objective] To develop a multiplex PCR method based on specific virulence-associated genes of SS4 virulent strains to achieve precise identification of such strains. [Methods] Based on previous research results, four target genes specific to SS4 virulent strains—sly, igdE, tran, and sao—were selected, and the SS4 serotype-specific gene wzy was used as an internal reference gene to design a pentaplex PCR method. After optimization of the multiplex PCR amplification system, specificity and sensitivity tests were conducted. This method was then employed to detect newly isolated SS4 strains. Additionally, zebrafish virulence assays were performed to validate the accuracy of this method. [Results] The multiplex PCR method specifically amplified the target genes, effectively distinguishing SS4 virulent strains from lowly virulent strains. The method exhibited high sensitivity, with a minimum detection limit of 4.1×102 CFU or 12.5 pg of genomic DNA. Specificity validation confirmed that this method accurately identified SS4 virulent strains. This method was then employed to examine six clinically isolated SS4 strains. Three strains were identified as virulent, showing high pathogenicity in zebrafish and causing a mortality rate of 60.00%-86.67%. The other three strains were identified as lowly virulent strains, exhibiting low pathogenicity in zebrafish and causing a mortality rate of 0-6.67%. [Conclusion] A multiplex PCR method based on virulence-associated genes of S. suis was successfully developed, enabling accurate and sensitive identification of SS4 virulent strains. This method provides technical support for the early diagnosis and effective prevention and control of S. suis infections.

Streptococcus suis  /  serotype 4  /  virulent strains  /  multiplex PCR
Jinlu ZHU, Zeren PENG, Hongkun ZHUANG, Jie SUN, Zongfu WU. A multiplex PCR method for identification of virulent strains of Streptococcus suis serotype 4[J]. Acta Microbiologica Sinica, 2025 , 65 (8) : 3782 -3793 . DOI: 10.13343/j.cnki.wsxb.20250119
Funding
Less
  • National Key Research and Development Program of China(2023YFD1800503)
  • Shenzhen Science and Technology Program(KCXFZ20230731094003006)
Appendix
Less
Year 2025 volume 65 Issue 8
PDF
145
56
Cite this Article
BibTeX
Article Info
doi: 10.13343/j.cnki.wsxb.20250119
  • Receive Date：2025-02-19
  • Online Date：2026-02-06
  • Published：2025-08-04
Article Data
Affiliations
History
  • Received：2025-02-19
  • Accepted：2025-03-19
Funding
National Key Research and Development Program of China(2023YFD1800503)
Shenzhen Science and Technology Program(KCXFZ20230731094003006)
Affiliations
    1.College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, China
    2.Key Lab of Animal Bacteriology, Ministry of Agriculture and Rural Affairs, Nanjing, Jiangsu, China
    3.WOAH Reference Lab for Swine Streptococcosis, Nanjing, Jiangsu, China
    4.Animal and Plant Inspection and Quarantine Technology Centre of Shenzhen Customs, Shenzhen, Guangdong, China
    5.Guangdong Provincial Key Laboratory of Research on the Technology of Pig-breeding and Pig-disease Prevention, Guangdong Haid Institute of Animal Husbandry & Veterinary, Guangzhou, Guangdong, China

Corresponding:

References
Share
https://castjournals.cast.org.cn/joweb/wswxb/EN/10.13343/j.cnki.wsxb.20250119
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House