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Limosilactobacillus fermentum H3260 alleviates hyperuricemia by regulating the gut microbiota
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Wenzhen LIN1, Xiaomei LIU2, Songjian YUAN2, Kai QIAN4, Yuantao LI2, Zhikai LIN1, Wei XU2, 3, 4, Bangzhou ZHANG2, 3, 4, *
Acta Microbiologica Sinica | 2025, 65(8) : 3702 - 3720
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Acta Microbiologica Sinica | 2025, 65(8): 3702-3720
• Research Article •
Limosilactobacillus fermentum H3260 alleviates hyperuricemia by regulating the gut microbiota
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Wenzhen LIN1, Xiaomei LIU2, Songjian YUAN2, Kai QIAN4, Yuantao LI2, Zhikai LIN1, Wei XU2, 3, 4, Bangzhou ZHANG2, 3, 4, *
Affiliations
  • 1.Fujian Key Laboratory of Subtropical Plant Physiology and Biochemistry, Fujian Institute of Subtropical Botany, Xiamen, Fujian, China
  • 2.Xiamen Treatgut Biotechnology Co. , Ltd. , Xiamen, Fujian, China
  • 3.Jiangxi Treatgut Biotechnology Co. , Ltd. , Yichun, Jiangxi, China
  • 4.School of Clinical Medicine, Yichun University, Yichun, Jiangxi, China
Published: 2025-08-04 doi: 10.13343/j.cnki.wsxb.20250099
Outline
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[Objective] To investigate the in vitro uric acid degradation performance and physiological and biochemical characteristics of Limosilactobacillus fermentum H3260 isolated from human feces and examine the effects of this strain on the serum uric acid level and gut microbiota in the mouse model of hyperuricemia, providing scientific evidence for the development of functional food for the prevention and treatment of hyperuricemia. [Methods] HPLC and the uric acid production assay were employed to determine the abilities of the target strain to degrade uric acid, adenosine, and nucleosides and to inhibit xanthine oxidase. The probiotic characteristics of the strain were evaluated by antimicrobial sensitivity tests and in vitro tolerance tests. The uric acid-lowering effect of L. fermentum H3260 was verified by in vivo experiments. [Results] L. fermentum H3260 was screened out. The strain exhibited degradation rates of (86.84±0.03)% for uric acid, (60.84±2.21)% for adenine, and (100.00±0.00)% for nucleosides, along with an inhibition rate of 22.48% for xanthine oxidase. The strain was sensitive to seven common antibiotics, including erythromycin, ceftriaxone, penicillin G, and chloramphenicol. After treatment in 0.3% bile salt for 2.5 h, the bacterial count remained above 1.00×106 CFU/mL. Animal experiments showed that the strain significantly reduced uric acid, creatinine, and blood urea nitrogen in hyperuricemic mice and regulate the gut microbiota to alleviate hyperuricemia. [Conclusion] We successfully screened out a strain L. fermentum H3260 capable of efficiently degrading uric acid, adenosine, and nucleosides. The strain exhibited good physiological and biochemical characteristics in vitro and significantly improved hyperuricemia-related indicators and regulated the gut microbiota in vivo, showing potential as an elite strain for the prevention and treatment of hyperuricemia.

uric acid  /  purine nucleoside  /  Lactobacillus
Wenzhen LIN, Xiaomei LIU, Songjian YUAN, Kai QIAN, Yuantao LI, Zhikai LIN, Wei XU, Bangzhou ZHANG. Limosilactobacillus fermentum H3260 alleviates hyperuricemia by regulating the gut microbiota[J]. Acta Microbiologica Sinica, 2025 , 65 (8) : 3702 -3720 . DOI: 10.13343/j.cnki.wsxb.20250099
Funding
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  • Fujian Province University Industry University Research Joint Innovation Project(2022Y4007)
  • National Natural Science Foundation of China(U22A20376)
  • Xiamen Science and Technology Planning(3502Z20182011)
Appendix
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Year 2025 volume 65 Issue 8
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194
75
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Article Info
doi: 10.13343/j.cnki.wsxb.20250099
  • Receive Date：2025-02-13
  • Online Date：2026-02-06
  • Published：2025-08-04
Article Data
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History
  • Received：2025-02-13
  • Accepted：2025-03-21
Funding
Fujian Province University Industry University Research Joint Innovation Project(2022Y4007)
National Natural Science Foundation of China(U22A20376)
Xiamen Science and Technology Planning(3502Z20182011)
Affiliations
    1.Fujian Key Laboratory of Subtropical Plant Physiology and Biochemistry, Fujian Institute of Subtropical Botany, Xiamen, Fujian, China
    2.Xiamen Treatgut Biotechnology Co. , Ltd. , Xiamen, Fujian, China
    3.Jiangxi Treatgut Biotechnology Co. , Ltd. , Yichun, Jiangxi, China
    4.School of Clinical Medicine, Yichun University, Yichun, Jiangxi, China

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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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