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[Objective] In view of the low decomposition rate of rice straw in black soil fields of cold regions, it is crucial to isolate lignin-degrading bacteria adaptive to low temperatures to enhance the straw degradation efficiency. [Methods] Soil samples were collected in winter, and the bacterial strains capable of degrading lignin were isolated by the streak-plate method with sodium lignosulfonate as a sole carbon source. The degradation conditions was carried out through optimized by single factor experiment sand response surface methodology. [Results] Pseudomonas psychrophila BYAU-6 was isolated, exhibiting strong lignin-degrading activity in low-temperature environments (5-15 ℃). The culture conditions for strain screening were as follows: sodium lignosulfonate addition amount of 0.5 g/L, a peptone-to-yeast powder mass ratio of 5:1, initial pH 7.0, and a liquid loading volume of 80%. The optimal culture conditions for lignin degradation were determined as follows: sodium lignosulfonate addition amount of 0.3 g/L, a peptone-to-yeast powder mass ratio of 3.2:2.8, initial pH 5.3, and a liquid loading volume of 80%. Under these conditions, the lignin degradation rate increased from 12.33% to 15.78%, representing an increase of 21.9%. The results of the pot experiment showed that the control group (without inoculation) achieved a straw degradation rate of 27.0%, while the inoculation with strain BYAU-6 achieved a straw degradation rate of 37.5%, an increase of 38.89% compared with the control (P<0.05). [Conclusion] This study provides novel microbial resources for straw degradation in cold regions and valuable data for future research on lignin-degrading strains under low-temperature conditions.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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