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Isolation and identification of a new species of Rhizobium from Sesbania cannabina
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Yan CHEN1, 2, Fuqiang WANG2, 3, *, Yong LONG3, Luyan FAN3, Shumeng REN4, Xianwei SONG2, Xiaofeng CAO2, 3, *
Acta Microbiologica Sinica | 2025, 65(8) : 3301 - 3316
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Acta Microbiologica Sinica | 2025, 65(8): 3301-3316
• Microbiome in Black Soils •
Isolation and identification of a new species of Rhizobium from Sesbania cannabina
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Yan CHEN1, 2, Fuqiang WANG2, 3, *, Yong LONG3, Luyan FAN3, Shumeng REN4, Xianwei SONG2, Xiaofeng CAO2, 3, *
Affiliations
  • 1.School of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan, China
  • 2.Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China
  • 3.Hainan Seed Industry Laboratory, Sanya, Hainan, China
  • 4.Sanya Institute of Henan University, Sanya, Hainan, China
Published: 2025-08-04 doi: 10.13343/j.cnki.wsxb.20250060
Outline
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[Objective] To screening stress-tolerant and high-yielding rhizobia with growth-promoting effects on Sesbaniacannabina and provide rhizobia resources for efficient cultivation of S. cannabina in saline-alkali soil. [Methods] The culture method was used to isolate endophytic rhizobia from S. cannabina ‘Zhongkejing 1’. Based on 16S rRNA gene and whole genome sequencing, the strains were identified, and their stress tolerance and plant growth-promoting characteristics were evaluated. Their growth-promoting effects on the original host variety and other materials of S. cannabina were verified. [Results] The rhizobia isolated from the root nodule samples of S. cannabina ‘Zhongkejing 1’ were identified as a species belonging to Rhizobium. Based on the ANI and dDDH values of the whole genome sequence, the strain was identified as a new species of Rhizobium and named Rhizobium sesbaniae ZK1T. R. sesbaniae ZK1T can tolerate a NaCl concentration of 2.0% and survive within the range of pH 4.0-10.0, and it had the ability to dissolve organophosphorus compounds. Pot experiments were conducted to evaluate the effects of R. sesbaniae ZK1T on the growth and nodulation of different materials of S. cannabina. The results revealed that R. sesbaniae ZK1T promoted the growth and nodulation of these materials, while it had a more efficient symbiotic relationship with the host variety. [Conclusion] The isolated new species R. sesbaniae ZK1T plays a role in promoting the growth and nodulation of S. cannabina and can tolerate severe acid, alkali, and salt stress. The findings have important theoretical significance and a practical value for the efficient improvement of plant-microorganism interactions in marginal land.

Sesbania cannabina  /  rhizobia  /  isolation and identification  /  stress tolerance  /  marginal land
Yan CHEN, Fuqiang WANG, Yong LONG, Luyan FAN, Shumeng REN, Xianwei SONG, Xiaofeng CAO. Isolation and identification of a new species of Rhizobium from Sesbania cannabina[J]. Acta Microbiologica Sinica, 2025 , 65 (8) : 3301 -3316 . DOI: 10.13343/j.cnki.wsxb.20250060
Funding
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  • Innovational Fund for Scientific and Technological Personnel of Hainan Province(KJRC2023D11)
  • Hainan Seed Industry Laboratory Project(B23E10002)
  • Strategic Priority Research Program of Chinese Academy of Sciences(XDA28030000)
Appendix
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Year 2025 volume 65 Issue 8
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140
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Article Info
doi: 10.13343/j.cnki.wsxb.20250060
  • Receive Date：2025-01-21
  • Online Date：2026-02-06
  • Published：2025-08-04
Article Data
Affiliations
History
  • Received：2025-01-21
  • Accepted：2025-03-11
Funding
Innovational Fund for Scientific and Technological Personnel of Hainan Province(KJRC2023D11)
Hainan Seed Industry Laboratory Project(B23E10002)
Strategic Priority Research Program of Chinese Academy of Sciences(XDA28030000)
Affiliations
    1.School of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan, China
    2.Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China
    3.Hainan Seed Industry Laboratory, Sanya, Hainan, China
    4.Sanya Institute of Henan University, Sanya, Hainan, China

Corresponding:

*E-mail: WANG Fuqiang,
CAO Xiaofeng,
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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