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Construction and characterization of ∆ vraSR-lrgAB of Staphylococcus epidermidis
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Shuangjie SHANG1, Weiguo CHEN1, Xiaokui ZHANG1, Jianpeng ZHU1, Song BAI1, Xiaoting CHEN1, Youcong WU1, 2, *
Acta Microbiologica Sinica | 2025, 65(2) : 629 - 643
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Acta Microbiologica Sinica | 2025, 65(2): 629-643
• Research Article •
Construction and characterization of ∆ vraSR-lrgAB of Staphylococcus epidermidis
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Shuangjie SHANG1, Weiguo CHEN1, Xiaokui ZHANG1, Jianpeng ZHU1, Song BAI1, Xiaoting CHEN1, Youcong WU1, 2, *
Affiliations
  • 1 Integrated Lab of Pathogenic Biology, School of Basic Medical Sciences, Dali University, Dali, Yunnan, China
  • 2 Department of Medical Microbiology and Immunology, School of Basic Medical Sciences, Dali University, Dali, Yunnan, China
Published: 2025-02-04 doi: 10.13343/j.cnki.wsxb.20240487
Outline
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[Objective] To explore the role of VraSR in regulating the biological functions of Staphylococcus epidermidisvia the CidA-LrgAB system. [Methods] The recombinant plasmid pKOR1-ΔlrgAB was constructed and then electroporated into SE1457 ∆vraSR to delete lrgAB from the genome of ∆vraSR by homologous recombination. The suspected mutant ∆vraSR-lrgAB was verified by PCR, RT-PCR, and sequencing. The growth, drug susceptibility, autolysis, and biofilm formation of ∆vraSR-lrgAB were determined. [Results] The S. epidermidis mutant ∆vraSR-lrgAB was successfully constructed. Compared with SE1457, ∆vraSR, and ∆lrgAB, ∆vraSR-lrgAB exhibited retarded growth, especially at 25 ℃ and 40 ℃ (P<0.001), increased drug susceptibility (P<0.01), enhanced autolysis (P<0.001), and reduced biofilm formation (P<0.01). [Conclusion] VraSR may regulate the growth, drug susceptibility, autolysis, and biofilm formation of S. epidermidis partly via the LrgAB system.

VraSR  /  LrgAB  /  homologous recombination  /  Staphylococcus epidermidis
Shuangjie SHANG, Weiguo CHEN, Xiaokui ZHANG, Jianpeng ZHU, Song BAI, Xiaoting CHEN, Youcong WU. Construction and characterization of ∆ vraSR-lrgAB of Staphylococcus epidermidis[J]. Acta Microbiologica Sinica, 2025 , 65 (2) : 629 -643 . DOI: 10.13343/j.cnki.wsxb.20240487
Funding
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  • National Natural Science Foundation of China(82060380)
  • National Natural Science Foundation of China(81660346)
  • Young and Middle-aged Academic Leader Training Foundation of Yunnan Province(202305AC160038)
Appendix
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Year 2025 volume 65 Issue 2
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98
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Article Info
doi: 10.13343/j.cnki.wsxb.20240487
  • Receive Date：2024-08-05
  • Online Date：2026-02-05
  • Published：2025-02-04
Article Data
Affiliations
History
  • Received：2024-08-05
  • Accepted：2024-11-15
Funding
National Natural Science Foundation of China(82060380)
National Natural Science Foundation of China(81660346)
Young and Middle-aged Academic Leader Training Foundation of Yunnan Province(202305AC160038)
Affiliations
    1 Integrated Lab of Pathogenic Biology, School of Basic Medical Sciences, Dali University, Dali, Yunnan, China
    2 Department of Medical Microbiology and Immunology, School of Basic Medical Sciences, Dali University, Dali, Yunnan, China

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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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