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Construction and biological characterization of greA/B-deleted Salmonella Typhimurium strains
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Jingfen YE1, 2, Shixiong CHEN1, 2, Shaobi WU1, 2, Wan YANG1, 2, Yong PAN3, Yiwen LIAO1, 2, Xue LUO1, 2, Qi YANG1, 2, *
Acta Microbiologica Sinica | 2025, 65(2) : 644 - 654
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Acta Microbiologica Sinica | 2025, 65(2): 644-654
• Research Article •
Construction and biological characterization of greA/B-deleted Salmonella Typhimurium strains
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Jingfen YE1, 2, Shixiong CHEN1, 2, Shaobi WU1, 2, Wan YANG1, 2, Yong PAN3, Yiwen LIAO1, 2, Xue LUO1, 2, Qi YANG1, 2, *
Affiliations
  • 1 School of Animal Science, Guizhou University, Guiyang, Guizhou, China
  • 2 Institute of Animal Diseases, Guizhou University, Guiyang, Guizhou, China
  • 3 Institute of Animal Husbandry and Veterinary Medicine, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou, China
Published: 2025-02-04 doi: 10.13343/j.cnki.wsxb.20240424
Outline
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Salmonella as common zoonotic pathogens can cause a variety of foodborne diseases. Salmonella Typhimurium (STM) is one of the key serotypes, and the research, prevention, and control of STM are of great significance to public health. [Objective] To investigate the effects of greA and greB on the biological characteristics and pathogenicity of STM. [Methods] Red homologous recombination was employed to construct the greA- and greB-deleted STM strains well as the complemented strains. The strains were then characterized in terms of growth characteristics, biofilm formation, and adhesion and invasion in Caco-2 cells. Mouse models were used to evaluate the effects of greA and greB deletion on STM pathogenicity. [Results] The mutant strains STM LT2ΔgreA and STM LT2ΔgreB were successfully constructed. Compared with the wild-type strain, the deletion of greA and greB did not affect the cell growth rate, while inhibiting the biofilm formation, adhesion, and invasion of STM. In addition, the deletion of greA and greB decreased the colonization of STM in the liver and spleen of mice, increasing the LD50 of STM by 39.81 times and 2.5 times, respectively. [Conclusion] The deletion of greA and greB could reduce the pathogenicity of STM. This finding provides a theoretical basis for further revealing the pathogenicity of Salmonella.

Salmonella Typhimurium  /  transcription elongation factor  /  greA  /  greB  /  biological characteristics
Jingfen YE, Shixiong CHEN, Shaobi WU, Wan YANG, Yong PAN, Yiwen LIAO, Xue LUO, Qi YANG. Construction and biological characterization of greA/B-deleted Salmonella Typhimurium strains[J]. Acta Microbiologica Sinica, 2025 , 65 (2) : 644 -654 . DOI: 10.13343/j.cnki.wsxb.20240424
Funding
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  • National Natural Science Foundation of China(32260884)
  • Guizhou Provincial Program on Commercialization of Scientific and Technological Achievements(黔科合支撑[2024]一般086号)
Appendix
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Year 2025 volume 65 Issue 2
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132
48
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Article Info
doi: 10.13343/j.cnki.wsxb.20240424
  • Receive Date：2024-07-13
  • Online Date：2026-02-05
  • Published：2025-02-04
Article Data
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History
  • Received：2024-07-13
  • Accepted：2024-11-15
Funding
National Natural Science Foundation of China(32260884)
Guizhou Provincial Program on Commercialization of Scientific and Technological Achievements(黔科合支撑[2024]一般086号)
Affiliations
    1 School of Animal Science, Guizhou University, Guiyang, Guizhou, China
    2 Institute of Animal Diseases, Guizhou University, Guiyang, Guizhou, China
    3 Institute of Animal Husbandry and Veterinary Medicine, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou, China

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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
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Genus		 种数
Number of
species		 占总种数比例
Percentage of total
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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