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Inhibition effect and transcriptome analysis of Gan Dan oral liquid against Vibrioparahaemolyticus
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Kuan CHU1, 2, 3, Lei HUANG2, Aqin ZHENG2, Xue CAI2, Anda CHENG4, Julin YUAN2, Liqun LYU1, 3, *, Jiayun YAO2, *
Acta Microbiologica Sinica | 2025, 65(2) : 683 - 697
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Acta Microbiologica Sinica | 2025, 65(2): 683-697
• Research Article •
Inhibition effect and transcriptome analysis of Gan Dan oral liquid against Vibrioparahaemolyticus
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Kuan CHU1, 2, 3, Lei HUANG2, Aqin ZHENG2, Xue CAI2, Anda CHENG4, Julin YUAN2, Liqun LYU1, 3, *, Jiayun YAO2, *
Affiliations
  • 1 National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai, China
  • 2 Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture and Rural Affairs, Key Laboratory of Fish Health and Nutrition of Zhejiang Province, Key Laboratory of Fishery Environment and Aquatic Product Quality and Safety of Huzhou City, Zhejiang Institute of Freshwater Fisheries, Huzhou, Zhejiang, China
  • 3 National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China
  • 4 Beijing Engineering Research Center of Chinese Traditional Veterinary Medicine, Beijing, China
Published: 2025-02-04 doi: 10.13343/j.cnki.wsxb.20240551
Outline
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[Objective] To investigate the invitro inhibitory activity of Gan Dan oral liquid (GD) against Vibrioparahaemolyticus and decipher the inhibition mechanism at the transcriptome level. [Methods] The invitro inhibitory activity of GD against V. parahaemolyticus was evaluated based on the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and growth curve. Scanning electron microscopy and transmission electron microscopy were employed to analyze the effect of GD on the cellular structure of V. parahaemolyticus. Transcriptome sequencing coupled with bioinformatics methods were employed to investigate the effect of GD at 1/4MIC on the transcriptome of V. parahaemolyticus, and the obtained results were examined by real-time quantitative reverse transcription PCR (RT-qPCR). [Results] GD exhibited high invitro inhibitory activity against V. parahaemolyticus, with the MIC and MBC of 7.6 mg/mL and 15.2 mg/mL, respectively. The GD treatment at a concentration of 1/4MIC disrupted the cell wall integrity and increased cell membrane permeability of the pathogen, and leading to the leakage of intracellular macromolecules. The results of transcriptome sequencing showed that GD treatment significantly altered the transcriptome profile of V. parahaemolyticus, resulting in significant upregulation of 1 074 genes and significant downregulation of 1 179 genes. The downregulated genes were mainly enriched in pathways related to the synthesis of inosinate and ribonucleoside, whereas the upregulated genes were primarily categorized into pathways associated with transcription factor activity and cell wall synthesis. [Conclusion] GD may inhibit V. parahaemolyticus by disrupting cell structure and inhibiting cellular energy metabolism and biosynthesis.

Vibrioparahaemolyticus  /  antibacterial drugs  /  cellular structure  /  antibacterial activity  /  transcriptome
Kuan CHU, Lei HUANG, Aqin ZHENG, Xue CAI, Anda CHENG, Julin YUAN, Liqun LYU, Jiayun YAO. Inhibition effect and transcriptome analysis of Gan Dan oral liquid against Vibrioparahaemolyticus[J]. Acta Microbiologica Sinica, 2025 , 65 (2) : 683 -697 . DOI: 10.13343/j.cnki.wsxb.20240551
Funding
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  • Zhejiang Technology Collaboration Project of “Jian Bing” “Ling Yan”(2024C02005)
  • Zhejiang Technology Collaboration Project of “Jian Bing” “Ling Yan”(2022C2027)
Appendix
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Year 2025 volume 65 Issue 2
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Article Info
doi: 10.13343/j.cnki.wsxb.20240551
  • Receive Date：2024-09-06
  • Online Date：2026-02-05
  • Published：2025-02-04
Article Data
Affiliations
History
  • Received：2024-09-06
  • Accepted：2024-11-18
Funding
Zhejiang Technology Collaboration Project of “Jian Bing” “Ling Yan”(2024C02005)
Zhejiang Technology Collaboration Project of “Jian Bing” “Ling Yan”(2022C2027)
Affiliations
    1 National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai, China
    2 Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture and Rural Affairs, Key Laboratory of Fish Health and Nutrition of Zhejiang Province, Key Laboratory of Fishery Environment and Aquatic Product Quality and Safety of Huzhou City, Zhejiang Institute of Freshwater Fisheries, Huzhou, Zhejiang, China
    3 National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China
    4 Beijing Engineering Research Center of Chinese Traditional Veterinary Medicine, Beijing, China

Corresponding:

*E-mail: LYU Liqun,
YAO Jiayun,
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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