PDF
Full
Outline
Phaseolus vulgaris L. is one of the key edible legumes in the world. Rhizosphere microorganisms have mutually beneficial interactions with plants, being important factors promoting crop growth and health. However, studies are limited regarding how to utilize the microbiomes of legumes to promote crop growth. [Objective] To investigate the structural and functional differences of microbial communities in the rhizosphere and root nodules between two varieties (‘Ziguan' and ‘Juguan') of P. vulgaris, screen rhizobial strains, and evaluate their nitrogen-fixing and growth-promoting properties. [Methods] We employed 16S rRNA gene sequencing to analyze the bacterial community structures in the rhizosphere and root nodules of the two varieties. The rhizobial strains were screened by the plate streaking method. Pot experiments with nitrogen-free vermiculite were carried out to evaluate the nitrogen-fixing performance of the 11 rhizobial strains screened out. [Results] The bacterial diversity in the rhizosphere soil of ‘Juguan' was significantly lower than that of ‘Ziguan', and the bacterial diversity in the rhizosphere soil samples of both varieties was significantly higher than that in the root nodule samples. In addition, the rhizosphere of P. vulgaris harbored beneficial bacterial genera such as Rhizobium, Sphingomonas, Burkholderia, among which Rhizobium was dominant in the root nodules of both varieties. Gephi network analysis showed that bacterial communities in the rhizosphere and root nodules had positive correlations, with the relative abundance of 75.52% and 86.67%, respectively. PICRUSt2 function prediction indicated that the bacteria in the rhizosphere mainly had the functions related to carbohydrate, amino acid, and lipid metabolism, with abundant genes involved in nitrogen metabolism. Pot experiments showed that Rhizobium lusitanum NZ5 and R. etli GLJ10 increased the underground dry weight of ‘Ziguan' by 43.21% and 48.15%, respectively. R. lusitanum NZ5, R. etli GLZ1, and R. changzhiense GLJ12 increased the underground dry weight of ‘Juguan' by 77.37%, 68.42%, and 67.37%, respectively. [Conclusion] P. vulgaris possesses ability to selectively enrich a variety of microorganisms in the soil, establishing a closely coordinated and highly functional microbial community in the rhizosphere. Moreover, different rhizobial strains exerted varied growth-promoting effects on P. vulgaris.
PDF
155
61
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
