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Influenza A/WSN/33 virus regulates the expression of orosomucoid through STAT3 phosphorylation mediated by TGF-β1/Smad3
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Dongxue YOU1, 2, 3, Chen PAN1, 2, Yuzhang CHEN1, 2, Xinni ZHOU1, 2, Ming GAO1, 3, Mengying CHEN1, 3, Jiajun WANG1, 3, Xiaojuan CHI1, 2, 3, Song WANG1, 2, 3, *
Acta Microbiologica Sinica | 2025, 65(2) : 771 - 780
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Acta Microbiologica Sinica | 2025, 65(2): 771-780
• Research Article •
Influenza A/WSN/33 virus regulates the expression of orosomucoid through STAT3 phosphorylation mediated by TGF-β1/Smad3
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Dongxue YOU1, 2, 3, Chen PAN1, 2, Yuzhang CHEN1, 2, Xinni ZHOU1, 2, Ming GAO1, 3, Mengying CHEN1, 3, Jiajun WANG1, 3, Xiaojuan CHI1, 2, 3, Song WANG1, 2, 3, *
Affiliations
  • 1 College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China
  • 2 Key Laboratory of Animal Pathogen Infection and Immunology of Fujian Province, Fuzhou, Fujian, China
  • 3 Key Laboratory of Fujian-Taiwan Animal Pathogen Biology, Fuzhou, Fujian, China
Published: 2025-02-04 doi: 10.13343/j.cnki.wsxb.20240549
Outline
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[Objective] To investigate the regulatory effect of transforming growth factor-beta 1 (TGF-β1)/Smad3 on orosomucoid (ORM) expression during infection with influenza A/WSN/33 virus (hereinafter referred to as WSN). [Methods] A549 cells were either infected with WSN or treated with TGF-β1, followed by treatment with Smad3 inhibitors. The mRNA levels of ORMs were assessed by RT-PCR. Subsequently, cell lines with signal transducer and activator of transcription 3 (STAT3) overexpression and knockdown were established, and RT-PCR and Western blotting were employed to evaluate the impact of STAT3 on ORM expression induced by the influenza virus. Furthermore, A549 cells underwent treatment with TGF-β1, after which the phosphorylation status of STAT3 was analyzed via Western blotting. Finally, the phosphorylation level of STAT3 was detected after inhibiting the activity of Smad3 or knocking down the expression of Smad3 in A549 cells infected with WSN or treated with TGF-β1. [Results] TGF-β1 regulated the expression of ORM1 and ORM2 through the activation of Smad3, a key mediator in the TGF-β signaling pathway. Concurrently, STAT3 was implicated in modulating ORM1 and ORM2 expression during WSN infection. Additionally, TGF-β1 was shown to induce STAT3 phosphorylation. Notably, inhibiting Smad3 activation or knocking down Smad3 expression suppressed STAT3 phosphorylation. [Conclusion] The regulation of ORM1 and ORM2 expression by WSN relied on STAT3 phosphorylation mediated by TGF-β1/Smad3.

influenza virus  /  TGF-β1/Smad3  /  signal transducer and activator of transcription 3 (STAT3)  /  orosomucoid
Dongxue YOU, Chen PAN, Yuzhang CHEN, Xinni ZHOU, Ming GAO, Mengying CHEN, Jiajun WANG, Xiaojuan CHI, Song WANG. Influenza A/WSN/33 virus regulates the expression of orosomucoid through STAT3 phosphorylation mediated by TGF-β1/Smad3[J]. Acta Microbiologica Sinica, 2025 , 65 (2) : 771 -780 . DOI: 10.13343/j.cnki.wsxb.20240549
Funding
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  • Natural Science Foundation of Fujian Province(2020J06016)
  • Science and Technology Innovation Project of Fujian Agriculture and Forestry University(KFb22063XA)
Appendix
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Year 2025 volume 65 Issue 2
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Article Info
doi: 10.13343/j.cnki.wsxb.20240549
  • Receive Date：2024-09-05
  • Online Date：2026-02-05
  • Published：2025-02-04
Article Data
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History
  • Received：2024-09-05
  • Accepted：2024-12-03
Funding
Natural Science Foundation of Fujian Province(2020J06016)
Science and Technology Innovation Project of Fujian Agriculture and Forestry University(KFb22063XA)
Affiliations
    1 College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China
    2 Key Laboratory of Animal Pathogen Infection and Immunology of Fujian Province, Fuzhou, Fujian, China
    3 Key Laboratory of Fujian-Taiwan Animal Pathogen Biology, Fuzhou, Fujian, China

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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
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Genus		 种数
Number of
species		 占总种数比例
Percentage of total
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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