PDF
Full
Outline
[Objective] To isolate the strain for solubilizing both inorganic and organic phosphorus (P) from the rhizosphere of Moso bamboo (Phyllostachys edulis) and mine the related genes, laying a foundation for activating the soil P pool and improving P bioavailability by P-solubilizing microorganisms (PSM). [Methods] High-throughput screening was employed to isolate PSM strains from the rhizosphere of Moso bamboo, an important economic plant in subtropical regions. The P-solubilizing activities of the strain under various carbon sources and soil P levels were investigated by microplate culture and soil inoculation experiments, respectively. The genes involved in P solubilizing of the strain were mined by whole-genome sequencing. [Results] The strain zafu-3 (Achromobacter xylosoxidans) capable of solubilizing P via multiple pathways was isolated from the rhizosphere of Moso bamboo. The strain solubilized Ca3(PO4)2, FePO4, AlPO4, lecithin, and calcium phytate by using four carbon sources such as glucose and citric acid, with the highest P-solubilizing activity of 32.75 mg/(L·d). Compared with the control group, the zafu-3 inoculation group showed increases of 4.21%-33.88% and 13.54%-112.06% in activities of acid phosphatase and alkaline phosphatase, respectively. In the high P-level soil, inoculation of the strain decreased the soil pH by 0.04 and increased the available P content by 16.32%. The whole genome of strain zafu-3 contained 35 genes encoding phosphohydrolases and 53 genes involved in organic acid metabolisms. Furthermore, strain zafu-3 carried the genes associated with the production of indole-3-acetic acid and siderophores, and the degradation of lignin. [Conclusion] Strain zafu-3 directly solubilized inorganic and organic P by secreting diverse organic acids and phosphatases, and indirectly activated soil P pools by promoting plant growth. These findings provide an important microbial resource and theoretical foundation for the development of multifunctional biofertilizers.
PDF
162
58
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
