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Isolation and identification of actinomycetes from rhizosphere soil samples of mangrove forests in Quanzhou Bay and secondary metabolites of Streptomyces sp. W444
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Wenzhou ZHANG1, Linlin CHEN1, Shuisen LIN1, Yangjun LIN1, Yue'e ZHUANG1, Zhixin XIE1, Hong JIANG2, Yunyang LIAN2, Zhuhua LUO3, Fei PENG1, *
Acta Microbiologica Sinica | 2025, 65(2) : 597 - 613
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Acta Microbiologica Sinica | 2025, 65(2): 597-613
• Research Article •
Isolation and identification of actinomycetes from rhizosphere soil samples of mangrove forests in Quanzhou Bay and secondary metabolites of Streptomyces sp. W444
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Wenzhou ZHANG1, Linlin CHEN1, Shuisen LIN1, Yangjun LIN1, Yue'e ZHUANG1, Zhixin XIE1, Hong JIANG2, Yunyang LIAN2, Zhuhua LUO3, Fei PENG1, *
Affiliations
  • 1 Quanzhou Medical College, Quanzhou, Fujian, China
  • 2 Fujian Institute of Microbiology, Fuzhou, Fujian, China
  • 3 Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, Fujian, China
Published: 2025-02-04 doi: 10.13343/j.cnki.wsxb.20240584
Outline
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[Objective] To isolate and identify actinomycetes from two mangrove soil samples in Quanzhou Bay as well as secondary metabolites from the target strain Streptomyces sp. W444 with antifungal activity. [Methods] The rhizosphere soil samples of two different mangrove plants were collected from Luoyang River in Quanzhou Bay. Actinomycetes were isolated from the soil samples by the dilution plating method. The isolates were classified by the phylogenetic tree constructed based on 16S rRNA gene sequences. The antifungal activities of the isolates were examined by the agar diffusion method. The target strain Streptomyces sp. W444 was subjected to fermentation scale-up for the isolation of secondary metabolites. Furthermore, the biosynthetic gene clusters were analyzed to deduce the biosynthetic pathway of staurosporine. [Results] A total of 56 strains of actinomycetes were isolated from mangrove soil samples and categorized into 8 genera belonging to 6 families of 6 orders. Among them, Streptomyces and Micromonospora were dominant, with the relative abundance of 41.0% and 33.9%, respectively. Strain Streptomyces sp. W444 exhibited excellent antifungal activity, and three indoles (staurosporine, K252c, and streptochlorin) were isolated and identified from this strain. The biosynthetic gene cluster of staurosporine was localized in the genome of Streptomyces sp. W444 by bioinformatics analysis. The biosynthetic pathway of staurosporine was then proposed. [Conclusion] Actinomycetes in the rhizosphere soil of mangrove plants in Quanzhou Bay had high diversity and contained potential natural product resources. Staurosporine, K252c, and streptochlorin were isolated from strain W444. These findings lay a solid foundation for studying the diversity and secondary metabolites of cultivable actinomycetes in mangrove forests in Quanzhou Bay.

mangrove in Quanzhou Bay  /  actinomycetes  /  indoles  /  biosynthetic gene cluster
Wenzhou ZHANG, Linlin CHEN, Shuisen LIN, Yangjun LIN, Yue'e ZHUANG, Zhixin XIE, Hong JIANG, Yunyang LIAN, Zhuhua LUO, Fei PENG. Isolation and identification of actinomycetes from rhizosphere soil samples of mangrove forests in Quanzhou Bay and secondary metabolites of Streptomyces sp. W444[J]. Acta Microbiologica Sinica, 2025 , 65 (2) : 597 -613 . DOI: 10.13343/j.cnki.wsxb.20240584
Funding
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  • Science and Technology Project of Quanzhou City(2023NS082)
  • Key Science and Technology Project of Quanzhou Medical College(XJK2210A)
  • Scientific Research Foundation of Third Institute of Oceanography, Ministry of Natural Resources(HY202304)
  • Research-based Teaching Project of Quanzhou Medical College(XJJK2308)
Appendix
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Year 2025 volume 65 Issue 2
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138
46
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Article Info
doi: 10.13343/j.cnki.wsxb.20240584
  • Receive Date：2024-09-23
  • Online Date：2026-02-05
  • Published：2025-02-04
Article Data
Affiliations
History
  • Received：2024-09-23
  • Accepted：2024-11-11
Funding
Science and Technology Project of Quanzhou City(2023NS082)
Key Science and Technology Project of Quanzhou Medical College(XJK2210A)
Scientific Research Foundation of Third Institute of Oceanography, Ministry of Natural Resources(HY202304)
Research-based Teaching Project of Quanzhou Medical College(XJJK2308)
Affiliations
    1 Quanzhou Medical College, Quanzhou, Fujian, China
    2 Fujian Institute of Microbiology, Fuzhou, Fujian, China
    3 Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, Fujian, China

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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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