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Research advances in CRISPR-based genetic editing of Bacillus subtilis
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Hanxuan GONG1, 2, Zhiwei WANG1, Yulin CHEN1, Yuxin YANG1, Gongwei LIU1
Acta Microbiologica Sinica | 2025, 65(5) : 1867 - 1884
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Acta Microbiologica Sinica | 2025, 65(5): 1867-1884
• Review •
Research advances in CRISPR-based genetic editing of Bacillus subtilis
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Hanxuan GONG1, 2, Zhiwei WANG1, Yulin CHEN1, Yuxin YANG1, Gongwei LIU1
Affiliations
  • 1.College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China
  • 2.Microbial Research Institute of Liaoning Province, Chaoyang, Liaoning, China
Published: 2025-05-04 doi: 10.13343/j.cnki.wsxb.20240688
Outline
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Bacillus subtilis is a generally recognized as safe (GRAS) probiotic and an excellent industrial chassis strain. It possesses advantages such as strong heterologous protein secretion capability, robust growth with low-quality carbon sources, and negligible codon bias. Since 2016, clustered regularly interspaced short palindromic repeats (CRISPR)-based gene editing has been successfully applied to B. subtilis, enabling precise genetic modifications, including point mutations, gene knockout, foreign gene insertion, gene expression regulation, and base editing. These advancements have significantly promoted the development of B. subtilis as an efficient microbial cell factory and have shown broad application potential in agriculture, pharmaceuticals, food production, and synthetic biology. This paper systematically review the development of the CRISPR system in B. subtilis and summarize its application in the efficient production of various products. The aim is to provide insights into the targeted optimization of metabolic pathways in B. subtilisvia the CRISPR system to achieve efficient and stable industrial production of target products, as well as to offer references for the further development and application of novel gene editing systems.

Bacillus subtilis  /  CRISPR  /  gene editing  /  gene expression regulation  /  synthetic biology
Hanxuan GONG, Zhiwei WANG, Yulin CHEN, Yuxin YANG, Gongwei LIU. Research advances in CRISPR-based genetic editing of Bacillus subtilis[J]. Acta Microbiologica Sinica, 2025 , 65 (5) : 1867 -1884 . DOI: 10.13343/j.cnki.wsxb.20240688
Funding
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  • National Key Research and Development Program of China(2022YFD1300201)
  • China Postdoctoral Science Foundation(2023M732893)
  • Shaanxi Postdoctoral Research Project(2023BSHEDZZ145)
  • China Agriculture Research System(CARS-39-12)
Appendix
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Year 2025 volume 65 Issue 5
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Article Info
doi: 10.13343/j.cnki.wsxb.20240688
  • Receive Date：2024-11-05
  • Online Date：2026-02-05
  • Published：2025-05-04
Article Data
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History
  • Received：2024-11-05
  • Accepted：2025-02-25
Funding
National Key Research and Development Program of China(2022YFD1300201)
China Postdoctoral Science Foundation(2023M732893)
Shaanxi Postdoctoral Research Project(2023BSHEDZZ145)
China Agriculture Research System(CARS-39-12)
Affiliations
    1.College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China
    2.Microbial Research Institute of Liaoning Province, Chaoyang, Liaoning, China

Corresponding:

E-mail: YANG Yuxin,
LIU Gongwei,
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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