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High light and nitrogen deprivation affect the response of Chlamydomonas reinhardtii to naphthenic acid stress
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Miao YANG1, Hengping LEI1, Ziyi YANG1, Meng WU1, Shiqi WEI1, Xi XIE2, Zheng GONG1
Acta Microbiologica Sinica | 2025, 65(5) : 2252 - 2266
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Acta Microbiologica Sinica | 2025, 65(5): 2252-2266
• Research Article •
High light and nitrogen deprivation affect the response of Chlamydomonas reinhardtii to naphthenic acid stress
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Miao YANG1, Hengping LEI1, Ziyi YANG1, Meng WU1, Shiqi WEI1, Xi XIE2, Zheng GONG1
Affiliations
  • 1.Key Laboratory of Plant Biotechnology of Liaoning Province, School of Life Science, Liaoning Normal University, Dalian, Liaoning, China
  • 2.Dalian Key Laboratory of Genetic Resources for Marine Shellfish, Key Laboratory of Conservation and Exploitation of Aquatic Germplasm Resource, Ministry of Agriculture and Rural Affairs, Liaoning Ocean and Fisheries Science Research Institute, Dalian, Liaoning, China
Published: 2025-05-04 doi: 10.13343/j.cnki.wsxb.20240613
Outline
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[Objective] To explore the physiochemical response mechanism of a model algal strain Chlamydomonas reinhardtii that hyper-accumulates oils to exposure of naphthenic acids (NAs). [Methods] The impacts of a typical NA, cyclohexanecarboxylic acid (CHCA), on the physiochemical parameters, including growth, photosynthetic activity, pH value of the culture, uptake of nitrogen and phosphorus, and biochemical constituents (lipids, carbohydrates, proteins, and pigments), of C. reinhardtii with high initial cell density under high light-nitrogen repletion (HL+N) and high light-nitrogen deprivation (HL-N) conditions were studied. [Results] The exposure to CHCA prominently promoted the uptake of phosphorus by C. reinhardtii under HL+N, while significantly inceasing the relative abundance of saturated C16:0 and decreasing the relative abundance of polyunsaturated C18:3n3. In contrast, CHCA treatment significantly inhibited the photosynthetic activity and phosphorus uptake but did not affect the fatty acid profile of C. reinhardtii under HL-N stress. In addition, the growth, pH value of the culture, and content of lipids, carbohydrates, proteins, and pigments, in C. reinhardtii under both HL+N and HL-N conditions all remained relatively constant when subjected to CHCA exposure. [Conclusion] The tolerance of microalgae to environmental stress can be visualized by growth curves, photosythetic activity, uptake of nitrogen and phosphorus, and key biochemical constituents. HL+N improves the tolerance of C. reinhardtii to NAs by promoting the uptake of phosphorus and altering the fatty acid profile, while the case was contrary under HL-N. These findings are beneficial for establishing strategies on effective cultivation of microalgae that highly tolerate NAs.

Chlamydomonas reinhardtii  /  naphthenic acid  /  high light and nitrogen deprivation  /  growth  /  photosynthetic activity  /  uptake of nitrogen and phosphorus  /  fatty acid biomarker
Miao YANG, Hengping LEI, Ziyi YANG, Meng WU, Shiqi WEI, Xi XIE, Zheng GONG. High light and nitrogen deprivation affect the response of Chlamydomonas reinhardtii to naphthenic acid stress[J]. Acta Microbiologica Sinica, 2025 , 65 (5) : 2252 -2266 . DOI: 10.13343/j.cnki.wsxb.20240613
Funding
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  • Basic Scientific Research Project of Education Department of Liaoning Province(LJKQZ20222365)
  • PhD Initiation Research Project of Liaoning Normal University(2022BSL009)
  • National Natural Science Foundation of China(32402984)
  • Special Funding for Basic Research Operating Expenses of Undergraduate Universities in Liaoning Province(LS2024Q004)
  • Horizontal Science and Technology Project of Liaoning Normal University(H2022011)
Appendix
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Year 2025 volume 65 Issue 5
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115
41
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Article Info
doi: 10.13343/j.cnki.wsxb.20240613
  • Receive Date：2024-10-08
  • Online Date：2026-02-05
  • Published：2025-05-04
Article Data
Affiliations
History
  • Received：2024-10-08
  • Accepted：2025-02-16
Funding
Basic Scientific Research Project of Education Department of Liaoning Province(LJKQZ20222365)
PhD Initiation Research Project of Liaoning Normal University(2022BSL009)
National Natural Science Foundation of China(32402984)
Special Funding for Basic Research Operating Expenses of Undergraduate Universities in Liaoning Province(LS2024Q004)
Horizontal Science and Technology Project of Liaoning Normal University(H2022011)
Affiliations
    1.Key Laboratory of Plant Biotechnology of Liaoning Province, School of Life Science, Liaoning Normal University, Dalian, Liaoning, China
    2.Dalian Key Laboratory of Genetic Resources for Marine Shellfish, Key Laboratory of Conservation and Exploitation of Aquatic Germplasm Resource, Ministry of Agriculture and Rural Affairs, Liaoning Ocean and Fisheries Science Research Institute, Dalian, Liaoning, China

Corresponding:

E-mail: XIE Xi,
GONG Zheng,
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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