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Recombinant expression of shRNA targeting gat in microalgae for controlling Aedes albopictus
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Xinxin TANG1, Xiaodong DENG2, 4, Xiaodan HUANG3, Siqi YANG1, Chunmei XUE1, Xiaowen FEI3
Acta Microbiologica Sinica | 2025, 65(5) : 2240 - 2251
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Acta Microbiologica Sinica | 2025, 65(5): 2240-2251
• Research Article •
Recombinant expression of shRNA targeting gat in microalgae for controlling Aedes albopictus
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Xinxin TANG1, Xiaodong DENG2, 4, Xiaodan HUANG3, Siqi YANG1, Chunmei XUE1, Xiaowen FEI3
Affiliations
  • 1.College of Biology and Agriculture, Jiamusi University, Jiamusi, Heilongjiang, China
  • 2.Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bioresources in Hainan Province, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, China
  • 3.School of Basic Medicine and Life Sciences, Hainan Medical University, Haikou, Hainan, China
  • 4.Zhanjiang Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong, China
Published: 2025-05-04 doi: 10.13343/j.cnki.wsxb.20240781
Outline
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[Objective] To explore the technical feasibility of using transgenic microalgae to control Aedes that transmit diseases such as dengue fever. [Methods] Taking the neurotransmitter transporter gamma-aminobutyric acid receptor gene (gat) of Aedes as a target, we constructed a shRNA-expressing vector and then transferred the vector into Chlamydomonas reinhardtiii CC124 and Chlorella vulgaris HOC5 through electroporation. The recombinant microalgae were used to feed the larvae and adults of Aedes albopictus. [Results] The transgenic algae delayed the larval development and had obvious lethal effects on the larvae and adults of A. albopictus, causing the mortality rates of 75.56% and 58.67%, respectively. The expression level of gat in the larvae was significantly down-regulated. [Conclusion] Deploying gat-shRNA transgenic microalgae in enclosed water environments to suppress mosquito populations in the vicinity is technically feasible. This strategy provides a new perspective on using biological methods to control mosquitoes and block the transmission of severe infectious diseases such as dengue fever and Zika virus disease.

Chlamydomonas reinhardtiii  /  Chlorella vulgaris  /  dengue fever  /  Aedes  /  gat
Xinxin TANG, Xiaodong DENG, Xiaodan HUANG, Siqi YANG, Chunmei XUE, Xiaowen FEI. Recombinant expression of shRNA targeting gat in microalgae for controlling Aedes albopictus[J]. Acta Microbiologica Sinica, 2025 , 65 (5) : 2240 -2251 . DOI: 10.13343/j.cnki.wsxb.20240781
Funding
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  • Key Research and Development Program of Hainan Province(ZDYF2022SHFZ314)
  • National Natural Science Foundation of China(82260669)
  • Natural Science Foundation of Hainan Province(322RC766)
  • Financial Special Project of the Ministry of Agriculture and Rural Affairs(NFZX2024)
Appendix
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Year 2025 volume 65 Issue 5
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154
65
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Article Info
doi: 10.13343/j.cnki.wsxb.20240781
  • Receive Date：2024-12-04
  • Online Date：2026-02-05
  • Published：2025-05-04
Article Data
Affiliations
History
  • Received：2024-12-04
  • Accepted：2024-12-31
Funding
Key Research and Development Program of Hainan Province(ZDYF2022SHFZ314)
National Natural Science Foundation of China(82260669)
Natural Science Foundation of Hainan Province(322RC766)
Financial Special Project of the Ministry of Agriculture and Rural Affairs(NFZX2024)
Affiliations
    1.College of Biology and Agriculture, Jiamusi University, Jiamusi, Heilongjiang, China
    2.Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bioresources in Hainan Province, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, China
    3.School of Basic Medicine and Life Sciences, Hainan Medical University, Haikou, Hainan, China
    4.Zhanjiang Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong, China

Corresponding:

E-mail: XUE Chunmei,
FEI Xiaowen,
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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