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Aeromonas hydrophila is a pathogen that can infect both fish and mammals, including humans. [Objective] To construct the tolA-deleted strain of A. hydrophila ATCC 7966 and use this strain to explore the biological functions of tolA. [Methods] We constructed the tolA-deleted strain AhΔtolA by homologous recombination and characterized the physiological phenotype of AhΔtolA. Transcriptome sequencing was performed to compare the gene expression between the wild type (WT) and AhΔtolA. [Results] The cell morphology of AhΔtolA was changed. The deletion of tolA significantly enhanced the sensitivity to sodium deoxycholate and oxidative stress, while significantly reducing the biofilm formation and the expression levels of several virulence genes. The yield of outer membrane vesicles was significantly increased in AhΔtolA. Transcriptomic analysis data showed that a total of 300 differentially expressed genes (DEGs) were screened between WT and AhΔtolA, including171 genes with up-regulated expression and 129 genes with down-regulated expression. GO enrichment analysis showed that the DEGs were mainly enriched in the oxidation-reduction process, metabolic process, outer membrane, and oxidoreductase activity. KEGG pathway enrichment analysis showed that the DEGs were mainly enriched in the biosynthesis of secondary metabolites, microbial metabolism in diverse environments, biosynthesis of cofactors, and biosynthesis of amino acids. [Conclusion] This study gives an insight into the roles of tolA in A. hydrophila and provides information about the metabolic pathways involving tolA. These results provide a theoretical reference for the prevention and control of A. hydrophila.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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