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Antifungal activity and its mechanism of Kobusin against Trichophyton interdigitale
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Xunyao ZHANG1, 2, 3, 4, Jiahao YUAN1, 2, 3, 4, Xiaowei PENG1, 2, 3, 4, Aijun LI1, 2, 3, 4, Gang YANG1, 2, 3, 4, Jianquan KAN1, 2, 3, 4
Acta Microbiologica Sinica | 2026, 66(2) : 723 - 738
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Acta Microbiologica Sinica | 2026, 66(2): 723-738
• Research Article •
Antifungal activity and its mechanism of Kobusin against Trichophyton interdigitale
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Xunyao ZHANG1, 2, 3, 4, Jiahao YUAN1, 2, 3, 4, Xiaowei PENG1, 2, 3, 4, Aijun LI1, 2, 3, 4, Gang YANG1, 2, 3, 4, Jianquan KAN1, 2, 3, 4
Affiliations
  • 1.College of Food Science, Southwest University, Chongqing, China
  • 2.Laboratory of Quality & Safety Risk Assessment for Agro-products on Storage and Preservation, Ministry of Agriculture and Rural Affairs, Chongqing, China
  • 3.China-Hungary Cooperative Research Centre for Food Science, Chongqing, China
  • 4.Chongqing Key Laboratory of Speciality Food Co-built by Sichuan and Chongqing, Chongqing, China
Published: 2026-02-04 doi: 10.13343/j.cnki.wsxb.20250621
Outline
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[Objective] To investigate the antifungal activity of Kobusin against Trichophyton interdigitale and its underlying mechanisms. [Methods] The minimal inhibitory concentration (MIC) of Kobusin was determined by the broth microdilution assay. The inhibitory effect of Kobusin on spore germination was observed microscopically, while that on hyphal radial growth was assessed on the agar plates containing Kobusin. Scanning electron microscopy (SEM) was employed to examine the morphological alterations in hyphae. Fluorescence microscopy and nucleic acid and protein leakage assays were employed to evaluated cell membrane integrity. Malvern Zetasizer was used to measure the changes in Zeta potential. A microplate reader was used to measure transmembrane potential, alkaline phosphatase (AKP) activity, malondialdehyde (MDA) content, reactive oxygen species (ROS) accumulation, superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) activities, mitochondrial membrane potential (MMP), ATP levels, as well as succinate dehydrogenase (SDH) and malate dehydrogenase (MDH) activities. [Results] Kobusin exhibited a MIC of 39 μg/mL against T. interdigitale, significantly inhibiting spore germination and hyphal growth. SEM revealed severe ultrastructural damage to hyphae. Fluorescence microscopy confirmed compromised membrane integrity, evidenced by increased nucleic acid and protein leakage and disrupted Zeta/transmembrane potentials. Meanwhile, Kobusin significantly increased the MDA content and ROS accumulation, inhibited the activities of AKP, SOD, CAT, and POD, markedly reduced MMP, decreased ATP synthesis, and weakened the activities of SDH and MDH. [Conclusion] Kobusin exerts antifungal effects by inhibiting spore germination and hyphal growth, disrupting cell membrane and cell wall integrity, interfering with membrane potential stability, inducing oxidative stress damage, and impairing mitochondrial energy metabolism.

Trichophyton interdigitale  /  Kobusin  /  antifungal mechanism
Xunyao ZHANG, Jiahao YUAN, Xiaowei PENG, Aijun LI, Gang YANG, Jianquan KAN. Antifungal activity and its mechanism of Kobusin against Trichophyton interdigitale[J]. Acta Microbiologica Sinica, 2026 , 66 (2) : 723 -738 . DOI: 10.13343/j.cnki.wsxb.20250621
Funding
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  • the Chongqing Science and Technology Bureau Under the Experiment and Demonstration Project(CSTB2023TIAD-KPX0031)
  • the Chongqing Agricultural and Rural Affairs Committee Project(CQMAITS05)
Appendix
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Year 2026 volume 66 Issue 2
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184
69
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Article Info
doi: 10.13343/j.cnki.wsxb.20250621
  • Receive Date：2025-08-10
  • Online Date：2026-02-05
  • Published：2026-02-04
Article Data
Affiliations
History
  • Received：2025-08-10
  • Accepted：2025-09-18
Funding
the Chongqing Science and Technology Bureau Under the Experiment and Demonstration Project(CSTB2023TIAD-KPX0031)
the Chongqing Agricultural and Rural Affairs Committee Project(CQMAITS05)
Affiliations
    1.College of Food Science, Southwest University, Chongqing, China
    2.Laboratory of Quality & Safety Risk Assessment for Agro-products on Storage and Preservation, Ministry of Agriculture and Rural Affairs, Chongqing, China
    3.China-Hungary Cooperative Research Centre for Food Science, Chongqing, China
    4.Chongqing Key Laboratory of Speciality Food Co-built by Sichuan and Chongqing, Chongqing, China

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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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