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Exploration of the diversity of culturable human intestinal microorganisms via multiple culture media
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Hao QI1, 2, 3, 4, Pei FU1, 2, 3, 4, Feiyan ZHAO1, 2, 3, 4, Wenjun LIU1, 2, 3, 4, Zhihong SUN1, 2, 3, 4
Acta Microbiologica Sinica | 2026, 66(2) : 783 - 800
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Acta Microbiologica Sinica | 2026, 66(2): 783-800
• Research Article •
Exploration of the diversity of culturable human intestinal microorganisms via multiple culture media
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Hao QI1, 2, 3, 4, Pei FU1, 2, 3, 4, Feiyan ZHAO1, 2, 3, 4, Wenjun LIU1, 2, 3, 4, Zhihong SUN1, 2, 3, 4
Affiliations
  • 1.Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China
  • 2.Collaborative Innovative Center of Lactic Acid Bacteria and Fermented Dairy Products, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China
  • 3.Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China
  • 4.Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China
Published: 2026-02-04 doi: 10.13343/j.cnki.wsxb.20250653
Outline
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[Objective] The human intestinal tract is rich in microbial resources, which play a significant role in the host’s digestion, absorption, growth, development, etc. Currently, culturomics is widely used in the isolation of beneficial intestinal microorganisms. However, different culture media have preferences, and a single medium is difficult to comprehensively isolate the culturable microorganisms in the intestinal tract. [Methods] We used six reported culture media [brain heart infusion (BHI), Wilkins_Chalgren anaerobe broth (WCBM), (Man-Rogosa-Sharpe) MRS, reinforced clostridial medium (RCM), mucin medium (MM) and modified mucin medium (MMM)] to isolate the microorganisms in the feces of 58 volunteers, with the aim of clarifying the diversity of culturable microorganisms in the intestinal tract and obtaining potential beneficial bacterial strains in the intestinal tract. [Results] A total of 1 052 bacterial strains were isolated from 58 samples, and they were identified as 101 species belonging to 39 genera of 5 phyla. The BHI medium isolated the most bacterial species (50, 49.50%), while the MMM medium isolated the fewest bacterial species (24, 23.76%). Except the MM medium, each of other media could isolate unique genera, and BHI and MMM isolated the most unique genera (5 each). Among the isolated strains, 466 strains were reported to have probiotic effects, including Bacteroides fragilis, Lactiplantibacillus plantarum, Pediococcus acidilactici, and Bifidobacterium bifidum. BHI and MRS media could isolate more beneficial microbial species (10/16, 62.50%). [Conclusion] We explored the diversity of beneficial bacteria in the human intestinal tract from the perspective of pure culture by using multiple culture media, providing rich strain resources for the development of intestinal beneficial microorganisms.

gut microbiota  /  isolation and identification  /  multiple culture media
Hao QI, Pei FU, Feiyan ZHAO, Wenjun LIU, Zhihong SUN. Exploration of the diversity of culturable human intestinal microorganisms via multiple culture media[J]. Acta Microbiologica Sinica, 2026 , 66 (2) : 783 -800 . DOI: 10.13343/j.cnki.wsxb.20250653
Funding
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  • the National Key Research and Development Program of China(2024YFA1307002)
Appendix
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Year 2026 volume 66 Issue 2
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Article Info
doi: 10.13343/j.cnki.wsxb.20250653
  • Receive Date：2025-08-25
  • Online Date：2026-02-05
  • Published：2026-02-04
Article Data
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History
  • Received：2025-08-25
  • Accepted：2025-09-27
Funding
the National Key Research and Development Program of China(2024YFA1307002)
Affiliations
    1.Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China
    2.Collaborative Innovative Center of Lactic Acid Bacteria and Fermented Dairy Products, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China
    3.Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China
    4.Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China

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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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