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cAMP receptor protein enhances Acinetobacter baumannii virulence by regulating cas3 expression
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Jiayuan HUANG1, Xinyue HUANG1, Ting YU2, Wenjie YUAN2, Ping CHEN3, Jian HU4, Jun XIE3, Guocai LI1, 5, 6
Acta Microbiologica Sinica | 2026, 66(2) : 867 - 880
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Acta Microbiologica Sinica | 2026, 66(2): 867-880
• Research Article •
cAMP receptor protein enhances Acinetobacter baumannii virulence by regulating cas3 expression
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Jiayuan HUANG1, Xinyue HUANG1, Ting YU2, Wenjie YUAN2, Ping CHEN3, Jian HU4, Jun XIE3, Guocai LI1, 5, 6
Affiliations
  • 1.School of Basic Medical Sciences & School of Public Health, Faculty of Medicine, Yangzhou University, Yangzhou, Jiangsu, China
  • 2.The First School of Clinical Medicine, Faculty of Medicine, Yangzhou University, Yangzhou, Jiangsu, China
  • 3.Xuyi People’s Hospital (Xuyi Clinical College Affiliated to Faculty of Medicine, Yangzhou University), Xuyi, Jiangsu, China
  • 4.Yixing Traditional Chinese Medicine Hospital (Yixing Traditional Chinese Medicine Hospital Affiliated to Faculty of Medicine, Yangzhou University), Yixing, Jiangsu, China
  • 5.Guangling College, Yangzhou University, Yangzhou, Jiangsu, China
  • 6.The Key Laboratory of the Jiangsu Higher Education Institutions for Nucleic Acid & Cell Fate Regulation, Yangzhou University, Yangzhou, Jiangsu, China
Published: 2026-02-04 doi: 10.13343/j.cnki.wsxb.20250689
Outline
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[Objective] To investigate the regulatory effect of the cAMP receptor protein (CRP), a transcription factor in Acinetobacter baumannii, on the cas3 gene. [Methods] CRP was expressed and purified via a prokaryotic expression system. EMSA was employed to examine CRP binding to the cas3 promoter. qPCR was conducted to evaluate the regulatory effect of CRP on cas3 expression. To further confirm the regulatory function of CRP, we constructed a mutant strain Δcrp. The impact of crp deletion on A. baumannii virulence was then analyzed via the biofilm formation assay, adhesion and invasion assays with A549 cells, a Galleria mellonella model, and a murine model of bacterial infection. [Results] EMSA demonstrated that CRP specifically bound to the cas3 promoter. The qPCR results showed that cas3 transcription was downregulated (P<0.001) in Δcrp. Compared with the wild-type strain, Δcrp exhibited no significant difference in growth capacity but enhanced biofilm formation (P<0.001) as well as strengthened adhesion (P=0.003<0.050) and invasion (P<0.001) in A549 cells. Furthermore, Δcrp demonstrated a markedly increased lethality rate in G. mellonella within 72 h. Furthermore, the murine infection experiment revealed that Δcrp possessed higher colonization capacity in the lungs than the wild-type strain (P<0.001). [Conclusion] CRP acts as a transcriptional activator that directly binds to the cas3 promoter to activate its transcription, thereby attenuating the virulence and pathogenicity of A. baumannii.

Acinetobacter baumannii  /  cAMP receptor protein  /  cas3  /  virulence
Jiayuan HUANG, Xinyue HUANG, Ting YU, Wenjie YUAN, Ping CHEN, Jian HU, Jun XIE, Guocai LI. cAMP receptor protein enhances Acinetobacter baumannii virulence by regulating cas3 expression[J]. Acta Microbiologica Sinica, 2026 , 66 (2) : 867 -880 . DOI: 10.13343/j.cnki.wsxb.20250689
Funding
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  • the National Natural Science Foundation of China(82373637)
  • the Natural Science Foundation of Jiangsu Province(BK20231241)
  • the Jiangsu Commission of Health Science Foundation(ZQ2024025)
  • the Yangzhou City Key Research and Development Project(YZ2023104)
  • the Wuxi Commission of Health Science Foundation(M202424)
Appendix
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Year 2026 volume 66 Issue 2
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Article Info
doi: 10.13343/j.cnki.wsxb.20250689
  • Receive Date：2025-09-08
  • Online Date：2026-02-05
  • Published：2026-02-04
Article Data
Affiliations
History
  • Received：2025-09-08
  • Accepted：2025-10-22
Funding
the National Natural Science Foundation of China(82373637)
the Natural Science Foundation of Jiangsu Province(BK20231241)
the Jiangsu Commission of Health Science Foundation(ZQ2024025)
the Yangzhou City Key Research and Development Project(YZ2023104)
the Wuxi Commission of Health Science Foundation(M202424)
Affiliations
    1.School of Basic Medical Sciences & School of Public Health, Faculty of Medicine, Yangzhou University, Yangzhou, Jiangsu, China
    2.The First School of Clinical Medicine, Faculty of Medicine, Yangzhou University, Yangzhou, Jiangsu, China
    3.Xuyi People’s Hospital (Xuyi Clinical College Affiliated to Faculty of Medicine, Yangzhou University), Xuyi, Jiangsu, China
    4.Yixing Traditional Chinese Medicine Hospital (Yixing Traditional Chinese Medicine Hospital Affiliated to Faculty of Medicine, Yangzhou University), Yixing, Jiangsu, China
    5.Guangling College, Yangzhou University, Yangzhou, Jiangsu, China
    6.The Key Laboratory of the Jiangsu Higher Education Institutions for Nucleic Acid & Cell Fate Regulation, Yangzhou University, Yangzhou, Jiangsu, China

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表12种不同金属材料的力学参数
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Family		 属数
Number of
genus		 种数
Number of
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Percentage of
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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