PDF
Full
Outline
Monascus, as a genus of edible fungi used in fermentation, are widely used in various industries such as wine making, food colorants, and pharmaceuticals due to their abundant secondary metabolites. McrA, a global regulator discovered in Aspergillus nidulans, has the function of regulating the growth and secondary metabolism of filamentous fungi. We had identified and cloned mcrA in Monascus purpureus in the previous study. [Objective] On the basis of transcriptome analysis, we mined the differentially expressed genes (DEGs) of ΔmcrA and trpC:mcrA strains to explore the function of mcrA. [Methods] The knockout strain ΔmcrA and overexpression strain trpC:mcrA of M. purpureus were constructed by homologous recombination. The colonies and microscopic morphology on different media were observed. The yields of Monascus pigments and citrinin were determined. The metabolic pathways involving DEGs were analyzed by transcriptome sequencing. [Results] The yields of Monascus pigments and citrinin of ΔmcrA decreased. Transcriptome sequencing results showed that the ΔmcrA strain up-regulated 111 genes and down-regulated 47 genes. The metabolic pathways involving the DEGs of ΔmcrA were mainly glycolysis, pyruvate metabolism, fatty acid synthesis, tyrosine metabolism and so on. The trpC:mcrA strain up-regulated 1 199 genes and down-regulated 867 genes. The main metabolic pathways involving the DEGs of trpC:mcrA were tryptophan metabolism, sucrose and starch metabolism, arginine and proline metabolism, fatty acid degradation, etc. [Conclusion] McrA is a global transcriptional regulator, and the knockout and overexpression of its gene will affect carbohydrate, lipid, and amino acid-related metabolic pathways, thus affecting the production of secondary metabolites.
PDF
225
90
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
