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Objective To investigate the effects of changes in single factors such as pH, metal ions, and NaCl concentration in the MG medium on the growth of Virgibacillus salexigens DSM 11483 and the yield of 5-hydroxyectoine (5-HE), optimize the culture conditions, and explore the mixed culture conditions of Halomonas campaniensis XH26 with V. salexigens DSM 11483 and the change in the yield of 5-HE. Methods We used a spectrophotometer was used to monitor the biomass (OD600) of bacteria. The HPLC method was used to measure the yields of ectoine and 5-HE. The one-sample t-test was conducted to analyze the difference significance of the experimental data. Results The optimal culture conditions for V. salexigens DSM 11483 were as follows: pH 9.0, FeSO4·7H2O concentration of 1.00 mmol/L, and NaCl concentration of 2.0 mol/L. In this medium, the yields of ectoine and 5-HE were 200.4 mg/L and 80.0 mg/L, respectively. After “feeding” V. salexigens DSM 11483 with 40.0 mmol/L ectoine and culturing for 72 h, the yield of 5-HE reached 1 373.5 mg/L, which was 17.2 times that in the control group. After 72 h of mixed culture of H. campaniensis XH26 cultured for 24 h with V. salexigens DSM 11483 cultured for 36 h, the yields of ectoine and 5-HE were 1 535.1 mg/L and 168.7 mg/L, respectively, which were 7.7 times and 2.1 times the yield of V. salexigens DSM 11483 in pure culture. Conclusions The mixed culture of halophilic bacteria can make full use of their respective advantages to improve the synthesis and transformation efficiency of ectoine, demonstrating further research value.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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