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Objective Developing plant-microbe combined techniques is significant for addressing the problem of declining cropland quality in China and enriching the approaches for biological remediation of degraded soils. Methods The plant growth-promoting traits of Burkholderia sp. YQ9 were determined by assessing its cellulase, protease, and ammonia production. Subsequently, a pot experiment was conducted to evaluate the effects of different dilutions of Burkholderia sp. YQ9 inoculant and culture medium on the growth of white clover (Trifolium repens) and the physicochemical properties of the rhizosphere soil. Furthermore, high-throughput sequencing was employed to analyze the impacts of different treatments on the structure of the rhizosphere microbial community. Results Burkholderia sp. YQ9 exhibited plant growth-promoting traits, being capable of producing cellulase, protease, and ammonia. White clover improved the pH environment of the rhizosphere soil. Application of the original inoculant of Burkholderia sp. YQ9 promoted white clover growth, significantly enhancing the content of soluble protein and soluble sugar in the shoots and leaves, as well as the levels of available phosphorus and available potassium in the rhizosphere soil, thereby facilitating organic matter decomposition. Analysis of microbial alpha diversity in the rhizosphere soil revealed that both the original inoculant of Burkholderia sp. YQ9 and the culture medium significantly reduced the richness, diversity, and evenness of both fungal and bacterial communities in the rhizosphere soil and altered the composition of the soil microbial community. The correlation analysis further indicated that the microbial community in the rhizosphere soil was correlated with white clover growth and soil physicochemical properties. Conclusion Burkholderia sp. YQ9 not only promoted the growth of white clover but also modified the composition of the rhizosphere microbial community and improved the soil fertility. These findings provide microbial augmentation-based technical support for enhancing cropland quality.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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