PDF
Full
Outline
Objective To address the environmental pollution caused by polyethylene terephthalate (PET), we screened functional bacterial strains capable of degrading PET and analyzed their growth and degradation characteristics, aiming to provide theoretical support and microbial resources for PET bioremediation. Methods Bacterial strains capable of degrading PET were isolated from the soil samples collected around a landfill site. The selected strain was identified based on morphological characteristics, physiological and biochemical properties, and 16S rRNA gene sequencing. The surface morphology and chemical group changes of PET films before and after degradation were analyzed by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and water contact angle (WCA) measurements. The types and concentrations of degradation products were quantified via high-performance liquid chromatography (HPLC). Results A PET-degrading strain, designated YH-1, was successfully isolated and identified as a member of the Cellulosimicrobium sp. genus based on 16S rRNA analysis. The optimal growth conditions for strain YH-1 were 35 °C, pH 7.0, and 1% salinity, and the strain exhibited robust growth within the ranges of pH 6.0-10.0 and 1%-4% salinity. After 6 days of incubation, YH-1 achieved a PET weight loss rate of 1.90%. HPLC revealed that terephthalic acid (TPA) and bis(2-hydroxyethyl) terephthalate (BHET) were the main degradation products, with concentrations of 3.87 mg/L and 4.70 mg/L, respectively. SEM images showed obvious surface roughening and cracking of the PET film after degradation, while FTIR revealed changes in functional groups. WCA measurements showed a reduction in contact angle from 79.385° to 65.052°, indicating enhanced hydrophilicity of the degraded PET film surface. Conclusion Strain YH-1 demonstrates good environmental adaptability and PET degradation potential. It can disrupt the PET film surface and generate typical degradation products. The findings lay a foundation for further development of PET-degrading microbial resources and exploration of degradation mechanisms.
PDF
326
136
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
